Comparison of analytical sensitivity of automated microbiological blood culture systems
Objective. To compare analytical parameters and sensitivity between BACT/ALERT® 3D 60 and Yunon® Labstar 50. Materials and Methods. The study included 20 species of clinically significant microorganisms isolated from blood (20 strains for each facultative anaerobic species and 10 strains for eac...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy
2024-09-01
|
| Series: | Клиническая микробиология и антимикробная химиотерапия |
| Subjects: | |
| Online Access: | https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p384/cmac-2024-t26-n3-p384.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841539843965845504 |
|---|---|
| author | Lyamin A.V. Ismatullin D.D. Kozlov A.V. Alekseev D.V. Kayumov K.A. Bochkareva P.V. Antipov V.A. |
| author_facet | Lyamin A.V. Ismatullin D.D. Kozlov A.V. Alekseev D.V. Kayumov K.A. Bochkareva P.V. Antipov V.A. |
| author_sort | Lyamin A.V. |
| collection | DOAJ |
| description | Objective.
To compare analytical parameters and sensitivity between BACT/ALERT® 3D 60 and Yunon® Labstar 50.
Materials and Methods.
The study included 20 species of clinically significant microorganisms isolated from blood (20 strains for each facultative anaerobic species and 10 strains for each aerobic species). For each strain, individual volumes of the inoculation dose were selected with the control of CFU value. In the case of obtaining a stable CFU value in the range 2–10 per 100 µl of bacterial cell suspension, 100 µl of suspension was inoculated into vials with nutrient media for each analyzer, followed by an analysis of the cultivation time, the presence or absence of a growth signal, and average CFU values. Aliquots of the vials contents were also inoculated onto solid nutrient media to evaluate false positive and false negative results.
Results.
It was revealed that the main analytical parameters of both devices were comparable. In addition, for two blood culture systems, the maximum limit value of CFU per sample was set, at which the probability of the growth signal absence from both analyzers significantly increased – 2 CFU per vial. At this value and values below, the analyzers did not detect the growth of microorganisms significantly more often. Also, a comparable median inoculation dose of 5 CFU per vial was established for both devices.
Conclusions.
The devices included in the study have comparable analytical parameters and allow to obtain a positive growth signal after inoculating material containing < 10 CFU per vial. However, it is necessary to consider increased probability of a negative result in case of inoculation dose equal to CFU ≤ 2. |
| format | Article |
| id | doaj-art-d96b1b1e3e0c48fca5b2b0d251c9fc79 |
| institution | Kabale University |
| issn | 1684-4386 2686-9586 |
| language | Russian |
| publishDate | 2024-09-01 |
| publisher | Interregional Association for Clinical Microbiology and Antimicrobial Chemotherapy |
| record_format | Article |
| series | Клиническая микробиология и антимикробная химиотерапия |
| spelling | doaj-art-d96b1b1e3e0c48fca5b2b0d251c9fc792025-01-14T06:35:36ZrusInterregional Association for Clinical Microbiology and Antimicrobial ChemotherapyКлиническая микробиология и антимикробная химиотерапия1684-43862686-95862024-09-0126338439210.36488/cmac.2024.3.384-392cmac-2024-t26-n3-p384Comparison of analytical sensitivity of automated microbiological blood culture systemsLyamin A.V.0Ismatullin D.D.1Kozlov A.V.2Alekseev D.V.3Kayumov K.A.4Bochkareva P.V.5Antipov V.A.6Professional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaProfessional Center for Education and Research in Genetic and Laboratory Technologies, Samara State Medical University, Samara, RussiaObjective. To compare analytical parameters and sensitivity between BACT/ALERT® 3D 60 and Yunon® Labstar 50. Materials and Methods. The study included 20 species of clinically significant microorganisms isolated from blood (20 strains for each facultative anaerobic species and 10 strains for each aerobic species). For each strain, individual volumes of the inoculation dose were selected with the control of CFU value. In the case of obtaining a stable CFU value in the range 2–10 per 100 µl of bacterial cell suspension, 100 µl of suspension was inoculated into vials with nutrient media for each analyzer, followed by an analysis of the cultivation time, the presence or absence of a growth signal, and average CFU values. Aliquots of the vials contents were also inoculated onto solid nutrient media to evaluate false positive and false negative results. Results. It was revealed that the main analytical parameters of both devices were comparable. In addition, for two blood culture systems, the maximum limit value of CFU per sample was set, at which the probability of the growth signal absence from both analyzers significantly increased – 2 CFU per vial. At this value and values below, the analyzers did not detect the growth of microorganisms significantly more often. Also, a comparable median inoculation dose of 5 CFU per vial was established for both devices. Conclusions. The devices included in the study have comparable analytical parameters and allow to obtain a positive growth signal after inoculating material containing < 10 CFU per vial. However, it is necessary to consider increased probability of a negative result in case of inoculation dose equal to CFU ≤ 2.https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p384/cmac-2024-t26-n3-p384.pdfbloodstream infectionsautomated blood cultures systemsblood cultures |
| spellingShingle | Lyamin A.V. Ismatullin D.D. Kozlov A.V. Alekseev D.V. Kayumov K.A. Bochkareva P.V. Antipov V.A. Comparison of analytical sensitivity of automated microbiological blood culture systems Клиническая микробиология и антимикробная химиотерапия bloodstream infections automated blood cultures systems blood cultures |
| title | Comparison of analytical sensitivity of automated microbiological blood culture systems |
| title_full | Comparison of analytical sensitivity of automated microbiological blood culture systems |
| title_fullStr | Comparison of analytical sensitivity of automated microbiological blood culture systems |
| title_full_unstemmed | Comparison of analytical sensitivity of automated microbiological blood culture systems |
| title_short | Comparison of analytical sensitivity of automated microbiological blood culture systems |
| title_sort | comparison of analytical sensitivity of automated microbiological blood culture systems |
| topic | bloodstream infections automated blood cultures systems blood cultures |
| url | https://cmac-journal.ru/publication/2024/3/cmac-2024-t26-n3-p384/cmac-2024-t26-n3-p384.pdf |
| work_keys_str_mv | AT lyaminav comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT ismatullindd comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT kozlovav comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT alekseevdv comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT kayumovka comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT bochkarevapv comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems AT antipovva comparisonofanalyticalsensitivityofautomatedmicrobiologicalbloodculturesystems |