A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose
This paper presents and successfully demonstrates a new form of passive Lab-on-printed circuit board (PCB) diagnostic platform for the quantification of glucose, as required for pumpless applications such as wearable diagnostic patches. The platform exploits copper oxide (CuO) nanoparticles for non-...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-10-01
|
| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137025000974 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849222542628225024 |
|---|---|
| author | Pavlos Menelaou Yujiang Zhu Anna Regoutz Despina Moschou |
| author_facet | Pavlos Menelaou Yujiang Zhu Anna Regoutz Despina Moschou |
| author_sort | Pavlos Menelaou |
| collection | DOAJ |
| description | This paper presents and successfully demonstrates a new form of passive Lab-on-printed circuit board (PCB) diagnostic platform for the quantification of glucose, as required for pumpless applications such as wearable diagnostic patches. The platform exploits copper oxide (CuO) nanoparticles for non-enzymatic, electrochemical glucose quantification, to achieve the high sensitivity and linear range of operation that is necessary for sweat or interstitial fluid sample analysis. As a result, the platform exhibits a low limit of detection (LoD) of 2.1 μM and a high sensitivity of 456 μA mM−1·cm−2, coupled with an excellent specificity against common glucose interfering species. The seamless integration of passive microfluidics and an electrochemical glucose biosensor is firstly outlined, and is fabricated using standard photolithography techniques in an up-scalable glucose quantification platform. The detection of glucose under two sample flow conditions is investigated in detail, including both static and dynamic conditions, revealing that when subject to a continuous flow the microsystem demonstrates an increase in sensitivity and a reduced linear range. This work demonstrates that our new passive Lab-on-printed circuit board (PCB) diagnostic platform can be successfully implemented under continuous sample flow conditions, and is therefore ideally suited to wearable diagnostic patch applications. In addition, the measured performance exceeds static flow approaches that have reported to date, including paper-based approaches. |
| format | Article |
| id | doaj-art-d8cda9be395043b0aa74e87ebe23bbe2 |
| institution | Kabale University |
| issn | 2590-1370 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj-art-d8cda9be395043b0aa74e87ebe23bbe22025-08-26T04:14:30ZengElsevierBiosensors and Bioelectronics: X2590-13702025-10-012610067010.1016/j.biosx.2025.100670A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucosePavlos Menelaou0Yujiang Zhu1Anna Regoutz2Despina Moschou3Electronic and Electrical Engineering, University of Bath, Bath, BA2 7AY, United Kingdom; Corresponding author.Department of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, United Kingdom; Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, OX1 3QR, United KingdomDepartment of Chemistry, University College London, 20 Gordon Street, London, WC1H 0AJ, United Kingdom; Department of Chemistry, University of Oxford, Inorganic Chemistry Laboratory, South Parks Road, OX1 3QR, United KingdomElectronic and Electrical Engineering, University of Bath, Bath, BA2 7AY, United KingdomThis paper presents and successfully demonstrates a new form of passive Lab-on-printed circuit board (PCB) diagnostic platform for the quantification of glucose, as required for pumpless applications such as wearable diagnostic patches. The platform exploits copper oxide (CuO) nanoparticles for non-enzymatic, electrochemical glucose quantification, to achieve the high sensitivity and linear range of operation that is necessary for sweat or interstitial fluid sample analysis. As a result, the platform exhibits a low limit of detection (LoD) of 2.1 μM and a high sensitivity of 456 μA mM−1·cm−2, coupled with an excellent specificity against common glucose interfering species. The seamless integration of passive microfluidics and an electrochemical glucose biosensor is firstly outlined, and is fabricated using standard photolithography techniques in an up-scalable glucose quantification platform. The detection of glucose under two sample flow conditions is investigated in detail, including both static and dynamic conditions, revealing that when subject to a continuous flow the microsystem demonstrates an increase in sensitivity and a reduced linear range. This work demonstrates that our new passive Lab-on-printed circuit board (PCB) diagnostic platform can be successfully implemented under continuous sample flow conditions, and is therefore ideally suited to wearable diagnostic patch applications. In addition, the measured performance exceeds static flow approaches that have reported to date, including paper-based approaches.http://www.sciencedirect.com/science/article/pii/S2590137025000974Copper oxideNon-enzymatic sensingGlucoseLab-on-PCBPassive microfluidicsElectrochemical biosensors |
| spellingShingle | Pavlos Menelaou Yujiang Zhu Anna Regoutz Despina Moschou A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose Biosensors and Bioelectronics: X Copper oxide Non-enzymatic sensing Glucose Lab-on-PCB Passive microfluidics Electrochemical biosensors |
| title | A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose |
| title_full | A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose |
| title_fullStr | A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose |
| title_full_unstemmed | A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose |
| title_short | A passive Lab-on-PCB microsystem for non-enzymatic quantification of glucose |
| title_sort | passive lab on pcb microsystem for non enzymatic quantification of glucose |
| topic | Copper oxide Non-enzymatic sensing Glucose Lab-on-PCB Passive microfluidics Electrochemical biosensors |
| url | http://www.sciencedirect.com/science/article/pii/S2590137025000974 |
| work_keys_str_mv | AT pavlosmenelaou apassivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT yujiangzhu apassivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT annaregoutz apassivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT despinamoschou apassivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT pavlosmenelaou passivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT yujiangzhu passivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT annaregoutz passivelabonpcbmicrosystemfornonenzymaticquantificationofglucose AT despinamoschou passivelabonpcbmicrosystemfornonenzymaticquantificationofglucose |