Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections
ABSTRACT Bloodstream infections (BSI) pose significant threats to patient health, necessitating timely and accurate diagnostics to reduce mortality and morbidity. This study aimed to evaluate the clinical performance of the BacT/ALERT VIRTUO blood culture system with FANPlus bottles compared to the...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2025-01-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01850-24 |
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| author | Yurong Qin Yiwen Liao Jingfang Zhou Weijiang Liu Huimin Chen Xiaoli Chen Weisha Wang Ni Zhang Yunhu Zhao Liang Wang Bing Gu Suling Liu |
| author_facet | Yurong Qin Yiwen Liao Jingfang Zhou Weijiang Liu Huimin Chen Xiaoli Chen Weisha Wang Ni Zhang Yunhu Zhao Liang Wang Bing Gu Suling Liu |
| author_sort | Yurong Qin |
| collection | DOAJ |
| description | ABSTRACT Bloodstream infections (BSI) pose significant threats to patient health, necessitating timely and accurate diagnostics to reduce mortality and morbidity. This study aimed to evaluate the clinical performance of the BacT/ALERT VIRTUO blood culture system with FANPlus bottles compared to the BACTEC FX400 system in detecting bloodstream pathogens. A total of 1,772 blood specimens were collected from various hospital wards. Specimen selection criteria were based on clinical suspicion of bloodstream infections, ensuring the inclusion of relevant and representative patient samples. These blood samples, collected from the same suspected sepsis patients, were cultured in parallel using both the BacT/ALERT VIRTUO and BACTEC FX400 systems. The diagnostic efficiency of both systems, including detection rates, time to detection (TTD), and sensitivity across different bacterial species, was assessed. In various application scenarios, the VIRTUO system demonstrates a higher positive detection rate, whether in the intensive care unit (ICU) (8.5% vs 6.4%, P = 0.028) or in general wards. Additionally, for different types of bacteria, the TVIRTUO system exhibits superior detection rates for anaerobic bacteria (5.9% vs 3.2%, P < 0.001) and aerobic bacteria (9.1% vs 7.2%, P = 0.043). Furthermore, it boasts a shorter median TTD of 14 hours compared to 16 hours, and a higher sensitivity for Gram-positive bacteria (2.8% vs 1.6%, P < 0.001). These findings emphasize VIRTUO’s effectiveness in enhancing diagnostic accuracy, achieving faster time to detection, and expanding the spectrum of detected organisms, thereby facilitating the quicker initiation of appropriate therapies, supporting precise clinical decision-making, and ultimately improving patient outcomes.IMPORTANCEOur study conducted a critical evaluation of advanced blood culture technologies for managing bloodstream infections (BSI). A distinctive strength of our research is the large sample size and the concurrent testing of the same patients with two systems, a methodology rarely achieved in other studies. BSIs present severe health threats, necessitating prompt and accurate diagnostics to mitigate morbidity and mortality. The BacT/ALERT VIRTUO system, in comparison to the BACTEC FX400 system, demonstrated superior detection capabilities, emphasizing the critical role of advanced diagnostics in clinical settings. |
| format | Article |
| id | doaj-art-d75d2b1db13c400ca87b66ebe75520a9 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-d75d2b1db13c400ca87b66ebe75520a92025-01-07T14:05:19ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-01-0113110.1128/spectrum.01850-24Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infectionsYurong Qin0Yiwen Liao1Jingfang Zhou2Weijiang Liu3Huimin Chen4Xiaoli Chen5Weisha Wang6Ni Zhang7Yunhu Zhao8Liang Wang9Bing Gu10Suling Liu11Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong, ChinaDepartment of Laboratory Medicine, Guangdong Provincial People‘s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, ChinaABSTRACT Bloodstream infections (BSI) pose significant threats to patient health, necessitating timely and accurate diagnostics to reduce mortality and morbidity. This study aimed to evaluate the clinical performance of the BacT/ALERT VIRTUO blood culture system with FANPlus bottles compared to the BACTEC FX400 system in detecting bloodstream pathogens. A total of 1,772 blood specimens were collected from various hospital wards. Specimen selection criteria were based on clinical suspicion of bloodstream infections, ensuring the inclusion of relevant and representative patient samples. These blood samples, collected from the same suspected sepsis patients, were cultured in parallel using both the BacT/ALERT VIRTUO and BACTEC FX400 systems. The diagnostic efficiency of both systems, including detection rates, time to detection (TTD), and sensitivity across different bacterial species, was assessed. In various application scenarios, the VIRTUO system demonstrates a higher positive detection rate, whether in the intensive care unit (ICU) (8.5% vs 6.4%, P = 0.028) or in general wards. Additionally, for different types of bacteria, the TVIRTUO system exhibits superior detection rates for anaerobic bacteria (5.9% vs 3.2%, P < 0.001) and aerobic bacteria (9.1% vs 7.2%, P = 0.043). Furthermore, it boasts a shorter median TTD of 14 hours compared to 16 hours, and a higher sensitivity for Gram-positive bacteria (2.8% vs 1.6%, P < 0.001). These findings emphasize VIRTUO’s effectiveness in enhancing diagnostic accuracy, achieving faster time to detection, and expanding the spectrum of detected organisms, thereby facilitating the quicker initiation of appropriate therapies, supporting precise clinical decision-making, and ultimately improving patient outcomes.IMPORTANCEOur study conducted a critical evaluation of advanced blood culture technologies for managing bloodstream infections (BSI). A distinctive strength of our research is the large sample size and the concurrent testing of the same patients with two systems, a methodology rarely achieved in other studies. BSIs present severe health threats, necessitating prompt and accurate diagnostics to mitigate morbidity and mortality. The BacT/ALERT VIRTUO system, in comparison to the BACTEC FX400 system, demonstrated superior detection capabilities, emphasizing the critical role of advanced diagnostics in clinical settings.https://journals.asm.org/doi/10.1128/spectrum.01850-24bloodstream infections (BSI)blood culture systemsdiagnostic accuracypathogen detectioncomparative analysisintensive care units (ICU) |
| spellingShingle | Yurong Qin Yiwen Liao Jingfang Zhou Weijiang Liu Huimin Chen Xiaoli Chen Weisha Wang Ni Zhang Yunhu Zhao Liang Wang Bing Gu Suling Liu Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections Microbiology Spectrum bloodstream infections (BSI) blood culture systems diagnostic accuracy pathogen detection comparative analysis intensive care units (ICU) |
| title | Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections |
| title_full | Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections |
| title_fullStr | Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections |
| title_full_unstemmed | Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections |
| title_short | Comparative evaluation of BacT/ALERT VIRTUO and BACTEC FX400 blood culture systems for the detection of bloodstream infections |
| title_sort | comparative evaluation of bact alert virtuo and bactec fx400 blood culture systems for the detection of bloodstream infections |
| topic | bloodstream infections (BSI) blood culture systems diagnostic accuracy pathogen detection comparative analysis intensive care units (ICU) |
| url | https://journals.asm.org/doi/10.1128/spectrum.01850-24 |
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