Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade
Severe burn injuries immediately trigger a sustained systemic and local stress response. During this process, the sympathetic nervous system releases large amounts of catecholamines, which bind to β-adrenergic receptors (β-AR) on cell membranes, negatively affecting skin regeneration. Additionally,...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004782 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841533339438153728 |
|---|---|
| author | Wenzhe Sun Hongwei Lu Pengqin Zhang Lian Zeng Bing Ye Yi Xu Jianan Chen Peiran Xue Jialin Yu Kaifang Chen Bin Wu Xiao Lv Xiaodong Guo Yanzhen Qu |
| author_facet | Wenzhe Sun Hongwei Lu Pengqin Zhang Lian Zeng Bing Ye Yi Xu Jianan Chen Peiran Xue Jialin Yu Kaifang Chen Bin Wu Xiao Lv Xiaodong Guo Yanzhen Qu |
| author_sort | Wenzhe Sun |
| collection | DOAJ |
| description | Severe burn injuries immediately trigger a sustained systemic and local stress response. During this process, the sympathetic nervous system releases large amounts of catecholamines, which bind to β-adrenergic receptors (β-AR) on cell membranes, negatively affecting skin regeneration. Additionally, recurrent bacterial infections make burn wounds difficult to treat, posing significant and ongoing challenges to burn care. To address these challenges, we pioneered the study of locally delivered propranolol for burn wound treatment, revealing its ability to antagonize norepinephrine (NE) and regulate the sympathetic nervous system. In this study, a Cu2⁺-loaded anti-sympathetic hydrogel (copper ion cross-linked propranolol@gelatin/alginate, PNL@GA-Cu) was developed to remodel the challenging neuromodulatory microenvironment and accelerate the repair of the infected burn wound. The hydrogel system releases Cu2⁺ and propranolol simultaneously during degradation, synergistically acting on local wound tissue. Cu2⁺ exhibits dual effects of antibacterial activity and promoting angiogenesis, effectively killing Staphylococcus aureus and Escherichia coli while enhancing the expression of angiogenesis-related genes (CD31, VEGF). Meanwhile, propranolol can counteract the inhibitory effects of NE simulated chronic stress microenvironment on angiogenesis and mitigate sympathetic nerve innervation during the early stages of wound healing. Finally, the PNL@GA-Cu hydrogel significantly promoted the repair of third-degree full-thickness burns in SD rats. Approaches targeting the neural microenvironment for burn wound treatment has not been previously addressed in the literature. The anti-sympathetic PNL@GA-Cu hydrogel offers a promising strategy for treating infected burn wounds. Remodeling the neuromodulatory microenvironment could be an emerging strategy in tissue engineering. |
| format | Article |
| id | doaj-art-d6e96dbf8b1849988f1503823fbfb1f5 |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-d6e96dbf8b1849988f1503823fbfb1f52025-01-17T04:52:09ZengElsevierMaterials Today Bio2590-00642025-02-0130101417Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockadeWenzhe Sun0Hongwei Lu1Pengqin Zhang2Lian Zeng3Bing Ye4Yi Xu5Jianan Chen6Peiran Xue7Jialin Yu8Kaifang Chen9Bin Wu10Xiao Lv11Xiaodong Guo12Yanzhen Qu13Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaDepartment of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaCorresponding author. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei Province, 430022, China.; Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaCorresponding author. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei Province, 430022, China.; Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaCorresponding author. Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, No. 1277 Jiefang Avenue, Wuhan, Hubei Province, 430022, China.; Department of Orthopaedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, ChinaSevere burn injuries immediately trigger a sustained systemic and local stress response. During this process, the sympathetic nervous system releases large amounts of catecholamines, which bind to β-adrenergic receptors (β-AR) on cell membranes, negatively affecting skin regeneration. Additionally, recurrent bacterial infections make burn wounds difficult to treat, posing significant and ongoing challenges to burn care. To address these challenges, we pioneered the study of locally delivered propranolol for burn wound treatment, revealing its ability to antagonize norepinephrine (NE) and regulate the sympathetic nervous system. In this study, a Cu2⁺-loaded anti-sympathetic hydrogel (copper ion cross-linked propranolol@gelatin/alginate, PNL@GA-Cu) was developed to remodel the challenging neuromodulatory microenvironment and accelerate the repair of the infected burn wound. The hydrogel system releases Cu2⁺ and propranolol simultaneously during degradation, synergistically acting on local wound tissue. Cu2⁺ exhibits dual effects of antibacterial activity and promoting angiogenesis, effectively killing Staphylococcus aureus and Escherichia coli while enhancing the expression of angiogenesis-related genes (CD31, VEGF). Meanwhile, propranolol can counteract the inhibitory effects of NE simulated chronic stress microenvironment on angiogenesis and mitigate sympathetic nerve innervation during the early stages of wound healing. Finally, the PNL@GA-Cu hydrogel significantly promoted the repair of third-degree full-thickness burns in SD rats. Approaches targeting the neural microenvironment for burn wound treatment has not been previously addressed in the literature. The anti-sympathetic PNL@GA-Cu hydrogel offers a promising strategy for treating infected burn wounds. Remodeling the neuromodulatory microenvironment could be an emerging strategy in tissue engineering.http://www.sciencedirect.com/science/article/pii/S2590006424004782Burn wound healingHydrogelAngiogenesisPropranololSympathetic nervesInfection |
| spellingShingle | Wenzhe Sun Hongwei Lu Pengqin Zhang Lian Zeng Bing Ye Yi Xu Jianan Chen Peiran Xue Jialin Yu Kaifang Chen Bin Wu Xiao Lv Xiaodong Guo Yanzhen Qu Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade Materials Today Bio Burn wound healing Hydrogel Angiogenesis Propranolol Sympathetic nerves Infection |
| title | Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade |
| title_full | Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade |
| title_fullStr | Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade |
| title_full_unstemmed | Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade |
| title_short | Localized propranolol delivery from a copper-loaded hydrogel for enhancing infected burn wound healing via adrenergic β-receptor blockade |
| title_sort | localized propranolol delivery from a copper loaded hydrogel for enhancing infected burn wound healing via adrenergic β receptor blockade |
| topic | Burn wound healing Hydrogel Angiogenesis Propranolol Sympathetic nerves Infection |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004782 |
| work_keys_str_mv | AT wenzhesun localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT hongweilu localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT pengqinzhang localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT lianzeng localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT bingye localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT yixu localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT jiananchen localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT peiranxue localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT jialinyu localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT kaifangchen localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT binwu localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT xiaolv localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT xiaodongguo localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade AT yanzhenqu localizedpropranololdeliveryfromacopperloadedhydrogelforenhancinginfectedburnwoundhealingviaadrenergicbreceptorblockade |