Efficient gene editing of pig embryos by combining electroporation and lipofection

Background and Aim: Mosaicism, which is characterized by the presence of wild-type and more than one mutant allele, poses a serious problem in zygotic gene modification through the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 system. Therefore, we use...

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Bibliographic Details
Main Authors: Qingyi Lin, Nanaka Torigoe, Bin Liu, Yuichiro Nakayama, Aya Nakai, Zhao Namula, Megumi Nagahara, Fuminori Tanihara, Maki Hirata, Takeshige Otoi
Format: Article
Language:English
Published: Veterinary World 2024-11-01
Series:Veterinary World
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Online Access:https://www.veterinaryworld.org/Vol.17/November-2024/30.pdf
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