Protocol to monitor activation of a two-component system in Dickeya dadantii during chicory leaf infection using Phos-tag gel

Summary: Quantifying the phosphorylation levels of proteins involved in bacterial signaling pathways is essential for understanding their role in stress adaptation and infection processes. Here, we present a protocol for the semi-quantitative assessment of the phosphorylation level of the transcript...

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Bibliographic Details
Main Authors: Edwige Madec, Jean-Marie Lacroix, Sébastien Bontemps-Gallo
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004058
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