Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves
Intracellular positional information is crucial for the precise control of biological phenomena, including cell division, polarity, and motility. Intracellular reaction-diffusion (iRD) waves are responsible for regulating positional information within cells as morphogens in multicellular tissues. Ho...
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| Format: | Article |
| Language: | English |
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The Biophysical Society of Japan
2024-11-01
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| Series: | Biophysics and Physicobiology |
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| Online Access: | https://doi.org/10.2142/biophysico.bppb-v21.0022 |
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| author | Sakura Takada Kei Fujiwara |
| author_facet | Sakura Takada Kei Fujiwara |
| author_sort | Sakura Takada |
| collection | DOAJ |
| description | Intracellular positional information is crucial for the precise control of biological phenomena, including cell division, polarity, and motility. Intracellular reaction-diffusion (iRD) waves are responsible for regulating positional information within cells as morphogens in multicellular tissues. However, iRD waves are explained by the coupling of biochemical reactions and molecular diffusion which indicates nonlinear systems under far from equilibrium conditions. Because of this complexity, experiments using defined elements rather than living cells containing endogenous factors are necessary to elucidate their pattern formation mechanisms. In this review, we summarize the effectiveness of artificial cell systems for investigating iRD waves derived from their high controllability and ability to emulate cell-size space effects. We describe how artificial cell systems reveal the characteristics of iRD waves, including the mechanisms of wave generation, mode selection, and period regulation. Furthermore, we introduce remaining open questions and discuss future challenges even in Min waves and in applying artificial cell systems to various iRD waves. |
| format | Article |
| id | doaj-art-d5c31533c1d94b40a64ef5e66fd2c251 |
| institution | Kabale University |
| issn | 2189-4779 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | The Biophysical Society of Japan |
| record_format | Article |
| series | Biophysics and Physicobiology |
| spelling | doaj-art-d5c31533c1d94b40a64ef5e66fd2c2512025-01-09T10:11:25ZengThe Biophysical Society of JapanBiophysics and Physicobiology2189-47792024-11-012110.2142/biophysico.bppb-v21.0022Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion wavesSakura Takada0Kei Fujiwara1Department of Biosciences and Informatics, Keio University, Yokohama, Kanagawa 223-8522, JapanDepartment of Biosciences and Informatics, Keio University, Yokohama, Kanagawa 223-8522, JapanIntracellular positional information is crucial for the precise control of biological phenomena, including cell division, polarity, and motility. Intracellular reaction-diffusion (iRD) waves are responsible for regulating positional information within cells as morphogens in multicellular tissues. However, iRD waves are explained by the coupling of biochemical reactions and molecular diffusion which indicates nonlinear systems under far from equilibrium conditions. Because of this complexity, experiments using defined elements rather than living cells containing endogenous factors are necessary to elucidate their pattern formation mechanisms. In this review, we summarize the effectiveness of artificial cell systems for investigating iRD waves derived from their high controllability and ability to emulate cell-size space effects. We describe how artificial cell systems reveal the characteristics of iRD waves, including the mechanisms of wave generation, mode selection, and period regulation. Furthermore, we introduce remaining open questions and discuss future challenges even in Min waves and in applying artificial cell systems to various iRD waves.https://doi.org/10.2142/biophysico.bppb-v21.0022bottom-up synthetic biologysynthetic cellreconstitutionspatiotemporal patterncell polarity |
| spellingShingle | Sakura Takada Kei Fujiwara Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves Biophysics and Physicobiology bottom-up synthetic biology synthetic cell reconstitution spatiotemporal pattern cell polarity |
| title | Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves |
| title_full | Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves |
| title_fullStr | Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves |
| title_full_unstemmed | Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves |
| title_short | Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves |
| title_sort | artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction diffusion waves |
| topic | bottom-up synthetic biology synthetic cell reconstitution spatiotemporal pattern cell polarity |
| url | https://doi.org/10.2142/biophysico.bppb-v21.0022 |
| work_keys_str_mv | AT sakuratakada artificialcellsystemasatoolforinvestigatingpatternformationmechanismsofintracellularreactiondiffusionwaves AT keifujiwara artificialcellsystemasatoolforinvestigatingpatternformationmechanismsofintracellularreactiondiffusionwaves |