A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model
Abstract Symbiotic gut microorganisms (microbiome) interact closely with the mammalian host's metabolism and are important determinants of human health. Here, we decipher the complex metabolic effects of microbial manipulation, by comparing germfree mice colonized by a human baby flora (HBF) or...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Springer Nature
2007-05-01
|
| Series: | Molecular Systems Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/msb4100153 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849225799174979584 |
|---|---|
| author | François‐Pierre J Martin Marc‐Emmanuel Dumas Yulan Wang Cristina Legido‐Quigley Ivan K S Yap Huiru Tang Séverine Zirah Gerard M Murphy Olivier Cloarec John C Lindon Norbert Sprenger Laurent B Fay Sunil Kochhar Peter van Bladeren Elaine Holmes Jeremy K Nicholson |
| author_facet | François‐Pierre J Martin Marc‐Emmanuel Dumas Yulan Wang Cristina Legido‐Quigley Ivan K S Yap Huiru Tang Séverine Zirah Gerard M Murphy Olivier Cloarec John C Lindon Norbert Sprenger Laurent B Fay Sunil Kochhar Peter van Bladeren Elaine Holmes Jeremy K Nicholson |
| author_sort | François‐Pierre J Martin |
| collection | DOAJ |
| description | Abstract Symbiotic gut microorganisms (microbiome) interact closely with the mammalian host's metabolism and are important determinants of human health. Here, we decipher the complex metabolic effects of microbial manipulation, by comparing germfree mice colonized by a human baby flora (HBF) or a normal flora to conventional mice. We perform parallel microbiological profiling, metabolic profiling by 1H nuclear magnetic resonance of liver, plasma, urine and ileal flushes, and targeted profiling of bile acids by ultra performance liquid chromatography–mass spectrometry and short‐chain fatty acids in cecum by GC‐FID. Top‐down multivariate analysis of metabolic profiles reveals a significant association of specific metabotypes with the resident microbiome. We derive a transgenomic graph model showing that HBF flora has a remarkably simple microbiome/metabolome correlation network, impacting directly on the host's ability to metabolize lipids: HBF mice present higher ileal concentrations of tauro‐conjugated bile acids, reduced plasma levels of lipoproteins but higher hepatic triglyceride content associated with depletion of glutathione. These data indicate that the microbiome modulates absorption, storage and the energy harvest from the diet at the systems level. |
| format | Article |
| id | doaj-art-d4a727dab6a644aead2f16d07d03fcc1 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2007-05-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-d4a727dab6a644aead2f16d07d03fcc12025-08-24T12:01:50ZengSpringer NatureMolecular Systems Biology1744-42922007-05-013111610.1038/msb4100153A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse modelFrançois‐Pierre J Martin0Marc‐Emmanuel Dumas1Yulan Wang2Cristina Legido‐Quigley3Ivan K S Yap4Huiru Tang5Séverine Zirah6Gerard M Murphy7Olivier Cloarec8John C Lindon9Norbert Sprenger10Laurent B Fay11Sunil Kochhar12Peter van Bladeren13Elaine Holmes14Jeremy K Nicholson15Department of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonNestlé Research CenterNestlé Research CenterNestlé Research CenterNestlé Research CenterDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonDepartment of Biomolecular Medicine, Division of Surgery, Oncology, Reproductive Biology and Anaesthetics, Faculty of Medicine, Imperial College LondonAbstract Symbiotic gut microorganisms (microbiome) interact closely with the mammalian host's metabolism and are important determinants of human health. Here, we decipher the complex metabolic effects of microbial manipulation, by comparing germfree mice colonized by a human baby flora (HBF) or a normal flora to conventional mice. We perform parallel microbiological profiling, metabolic profiling by 1H nuclear magnetic resonance of liver, plasma, urine and ileal flushes, and targeted profiling of bile acids by ultra performance liquid chromatography–mass spectrometry and short‐chain fatty acids in cecum by GC‐FID. Top‐down multivariate analysis of metabolic profiles reveals a significant association of specific metabotypes with the resident microbiome. We derive a transgenomic graph model showing that HBF flora has a remarkably simple microbiome/metabolome correlation network, impacting directly on the host's ability to metabolize lipids: HBF mice present higher ileal concentrations of tauro‐conjugated bile acids, reduced plasma levels of lipoproteins but higher hepatic triglyceride content associated with depletion of glutathione. These data indicate that the microbiome modulates absorption, storage and the energy harvest from the diet at the systems level.https://doi.org/10.1038/msb4100153co‐metabolismgut microflorametabonomics/metabolomicsnetwork modelingsystems biology |
| spellingShingle | François‐Pierre J Martin Marc‐Emmanuel Dumas Yulan Wang Cristina Legido‐Quigley Ivan K S Yap Huiru Tang Séverine Zirah Gerard M Murphy Olivier Cloarec John C Lindon Norbert Sprenger Laurent B Fay Sunil Kochhar Peter van Bladeren Elaine Holmes Jeremy K Nicholson A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model Molecular Systems Biology co‐metabolism gut microflora metabonomics/metabolomics network modeling systems biology |
| title | A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model |
| title_full | A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model |
| title_fullStr | A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model |
| title_full_unstemmed | A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model |
| title_short | A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model |
| title_sort | top down systems biology view of microbiome mammalian metabolic interactions in a mouse model |
| topic | co‐metabolism gut microflora metabonomics/metabolomics network modeling systems biology |
| url | https://doi.org/10.1038/msb4100153 |
| work_keys_str_mv | AT francoispierrejmartin atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT marcemmanueldumas atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT yulanwang atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT cristinalegidoquigley atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT ivanksyap atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT huirutang atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT severinezirah atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT gerardmmurphy atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT oliviercloarec atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT johnclindon atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT norbertsprenger atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT laurentbfay atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT sunilkochhar atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT petervanbladeren atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT elaineholmes atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT jeremyknicholson atopdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT francoispierrejmartin topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT marcemmanueldumas topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT yulanwang topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT cristinalegidoquigley topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT ivanksyap topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT huirutang topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT severinezirah topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT gerardmmurphy topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT oliviercloarec topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT johnclindon topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT norbertsprenger topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT laurentbfay topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT sunilkochhar topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT petervanbladeren topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT elaineholmes topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel AT jeremyknicholson topdownsystemsbiologyviewofmicrobiomemammalianmetabolicinteractionsinamousemodel |