Ironing out differences in attenuation and blooming artifact in acute stroke thrombi
Abstract This study aims to improve our understanding of acute ischemic stroke clot imaging by integrating CT attenuation information with MRI susceptibility signal of thrombi. For this proof-of-principle experimental study, fifty-seven clot analogs were produced using ovine venous blood with a broa...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83916-0 |
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| author | Aglae Velasco Gonzalez Dennis Görlich Boris Buerke Cristina Sauerland Norbert Meier Manfred Fobker Ray McCarthy Astrid Jeibmann Walter Heindel Andreas Faldum Harald Kugel |
| author_facet | Aglae Velasco Gonzalez Dennis Görlich Boris Buerke Cristina Sauerland Norbert Meier Manfred Fobker Ray McCarthy Astrid Jeibmann Walter Heindel Andreas Faldum Harald Kugel |
| author_sort | Aglae Velasco Gonzalez |
| collection | DOAJ |
| description | Abstract This study aims to improve our understanding of acute ischemic stroke clot imaging by integrating CT attenuation information with MRI susceptibility signal of thrombi. For this proof-of-principle experimental study, fifty-seven clot analogs were produced using ovine venous blood with a broad histological spectrum. Each clot analog was analyzed to determine its RBC content and chemical composition, including water, Fe III, sodium, pH, and pO2. Non-contrast CT and a susceptibility-weighted MRI sequence were used for imaging. The study found that RBC content correlated more accurately than iron content with clot attenuation on CT. There was a strong correlation between Fe III content and RBC percentage in clots. Specifically, changes in RBC content accounted for 64% of the variance in Fe III content (R2 = 0.640; p < .0001). Thrombi with blooming artifacts (BA) displayed higher attenuation on non-contrast CT than those without (73.4 vs. 40 HU, p < .0001) and had the highest RBC and iron contents. The cut-off value of 1242 µg/g of iron predicted blooming artifacts with high sensitivity and specificity. The pH level strongly affected the appearance of blooming artifacts, particularly for negative clots with high RBC content. These findings provide significant insights into the imaging behavior of acute ischemic stroke clots across both imaging modalities and could potentially improve the diagnosis and treatment of acute stroke patients. Furthermore, these results open the possibility for future research aimed at developing pH-modulated therapeutic strategies based on the acid-base state of thrombi. |
| format | Article |
| id | doaj-art-d3d7eaaafdeb452fac1f70b4b4c76f5c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-d3d7eaaafdeb452fac1f70b4b4c76f5c2025-01-05T12:22:51ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-024-83916-0Ironing out differences in attenuation and blooming artifact in acute stroke thrombiAglae Velasco Gonzalez0Dennis Görlich1Boris Buerke2Cristina Sauerland3Norbert Meier4Manfred Fobker5Ray McCarthy6Astrid Jeibmann7Walter Heindel8Andreas Faldum9Harald Kugel10Clinic for Radiology, Neuroradiology, University of Münster and University Hospital MünsterInstitute of Biostatistics and Clinical Research, University of MünsterClinic for Radiology, Neuroradiology, University of Münster and University Hospital MünsterInstitute of Biostatistics and Clinical Research, University of MünsterMedical Physics, Clinic for Radiology, University of Münster and University Hospital of MünsterCenter for Laboratory Medicine, University Hospital MünsterCerenovus, Galway Neuro Technology CentreInstitute of Neuropathology, University Hospital MünsterClinic for Radiology, Neuroradiology, University of Münster and University Hospital MünsterInstitute of Biostatistics and Clinical Research, University of MünsterMedical Physics, Clinic for Radiology, University of Münster and University Hospital of MünsterAbstract This study aims to improve our understanding of acute ischemic stroke clot imaging by integrating CT attenuation information with MRI susceptibility signal of thrombi. For this proof-of-principle experimental study, fifty-seven clot analogs were produced using ovine venous blood with a broad histological spectrum. Each clot analog was analyzed to determine its RBC content and chemical composition, including water, Fe III, sodium, pH, and pO2. Non-contrast CT and a susceptibility-weighted MRI sequence were used for imaging. The study found that RBC content correlated more accurately than iron content with clot attenuation on CT. There was a strong correlation between Fe III content and RBC percentage in clots. Specifically, changes in RBC content accounted for 64% of the variance in Fe III content (R2 = 0.640; p < .0001). Thrombi with blooming artifacts (BA) displayed higher attenuation on non-contrast CT than those without (73.4 vs. 40 HU, p < .0001) and had the highest RBC and iron contents. The cut-off value of 1242 µg/g of iron predicted blooming artifacts with high sensitivity and specificity. The pH level strongly affected the appearance of blooming artifacts, particularly for negative clots with high RBC content. These findings provide significant insights into the imaging behavior of acute ischemic stroke clots across both imaging modalities and could potentially improve the diagnosis and treatment of acute stroke patients. Furthermore, these results open the possibility for future research aimed at developing pH-modulated therapeutic strategies based on the acid-base state of thrombi.https://doi.org/10.1038/s41598-024-83916-0Blood clotHelical CTRed blood cellsDecision treesIron |
| spellingShingle | Aglae Velasco Gonzalez Dennis Görlich Boris Buerke Cristina Sauerland Norbert Meier Manfred Fobker Ray McCarthy Astrid Jeibmann Walter Heindel Andreas Faldum Harald Kugel Ironing out differences in attenuation and blooming artifact in acute stroke thrombi Scientific Reports Blood clot Helical CT Red blood cells Decision trees Iron |
| title | Ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| title_full | Ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| title_fullStr | Ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| title_full_unstemmed | Ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| title_short | Ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| title_sort | ironing out differences in attenuation and blooming artifact in acute stroke thrombi |
| topic | Blood clot Helical CT Red blood cells Decision trees Iron |
| url | https://doi.org/10.1038/s41598-024-83916-0 |
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