Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation
The multiscale hierarchical porous titanium scaffolds significantly improve biomechanical adaptation and biological activity by simulating bone structure, presenting attractive application prospects. To achieve the controllable preparation of multiscale hierarchical porous Ti-3Zr-2Sn-3Mo-25Nb (TLM)...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524009092 |
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| author | QingLian Yang Lan Wang WenHao Zhou JinYang Ge XiaoTong Lu HanYuan Liu Sen Yu |
| author_facet | QingLian Yang Lan Wang WenHao Zhou JinYang Ge XiaoTong Lu HanYuan Liu Sen Yu |
| author_sort | QingLian Yang |
| collection | DOAJ |
| description | The multiscale hierarchical porous titanium scaffolds significantly improve biomechanical adaptation and biological activity by simulating bone structure, presenting attractive application prospects. To achieve the controllable preparation of multiscale hierarchical porous Ti-3Zr-2Sn-3Mo-25Nb (TLM) scaffolds, this study innovatively proposed the combination of additive manufacturing and electrochemical dealloying. The macro-scale pores of TLM were fabricated using selective laser melting (SLM), with a pore size of 640 μm and a porosity of 70.92 %. Then, a biphasic microstructure was obtained by heat treatment. Finally, the micro- and nano-scale structure was prepared via electrochemical dealloying. The elastic modulus of the multiscale hierarchical porous TLM scaffolds was 1.43 GPa, which closely matched the human bone tissue, indicating excellent biomechanical adaptation. Meanwhile, in vitro experiments further demonstrated that these scaffolds significantly enhanced BMSCs adhesion. The alkaline phosphatase (ALP) activity, calcium deposition, and collagen secretion were up-regulated, while lipid differentiation was down-regulated, indicating the scaffolds exhibited the potential for osseointegration. This research provides a valuable method for the preparation of multiscale hierarchical porous titanium scaffolds and offers innovative insights for the development of customized hard-tissue implants. |
| format | Article |
| id | doaj-art-d2b8f91446124a10b56e337ee31e9d85 |
| institution | Kabale University |
| issn | 0264-1275 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-d2b8f91446124a10b56e337ee31e9d852025-01-09T06:12:19ZengElsevierMaterials & Design0264-12752025-01-01249113534Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiationQingLian Yang0Lan Wang1WenHao Zhou2JinYang Ge3XiaoTong Lu4HanYuan Liu5Sen Yu6School of Materials Science and Engineering, Northeastern University, Shenyang 110004, China; Shaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, ChinaShaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, China; Corresponding authors.Shaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, ChinaState Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, ChinaShaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, ChinaShaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, ChinaShaanxi Key Laboratory of Biomedical Metal Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, China; Corresponding authors.The multiscale hierarchical porous titanium scaffolds significantly improve biomechanical adaptation and biological activity by simulating bone structure, presenting attractive application prospects. To achieve the controllable preparation of multiscale hierarchical porous Ti-3Zr-2Sn-3Mo-25Nb (TLM) scaffolds, this study innovatively proposed the combination of additive manufacturing and electrochemical dealloying. The macro-scale pores of TLM were fabricated using selective laser melting (SLM), with a pore size of 640 μm and a porosity of 70.92 %. Then, a biphasic microstructure was obtained by heat treatment. Finally, the micro- and nano-scale structure was prepared via electrochemical dealloying. The elastic modulus of the multiscale hierarchical porous TLM scaffolds was 1.43 GPa, which closely matched the human bone tissue, indicating excellent biomechanical adaptation. Meanwhile, in vitro experiments further demonstrated that these scaffolds significantly enhanced BMSCs adhesion. The alkaline phosphatase (ALP) activity, calcium deposition, and collagen secretion were up-regulated, while lipid differentiation was down-regulated, indicating the scaffolds exhibited the potential for osseointegration. This research provides a valuable method for the preparation of multiscale hierarchical porous titanium scaffolds and offers innovative insights for the development of customized hard-tissue implants.http://www.sciencedirect.com/science/article/pii/S0264127524009092Multiscale hierarchical porous titanium scaffoldBiomechanical adaptationOsteogenic differentiationElectrochemical dealloying |
| spellingShingle | QingLian Yang Lan Wang WenHao Zhou JinYang Ge XiaoTong Lu HanYuan Liu Sen Yu Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation Materials & Design Multiscale hierarchical porous titanium scaffold Biomechanical adaptation Osteogenic differentiation Electrochemical dealloying |
| title | Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| title_full | Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| title_fullStr | Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| title_full_unstemmed | Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| title_short | Construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| title_sort | construction of multiscale hierarchical porous titanium scaffolds and in vitro evaluation of osteogenic differentiation |
| topic | Multiscale hierarchical porous titanium scaffold Biomechanical adaptation Osteogenic differentiation Electrochemical dealloying |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524009092 |
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