Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity
Abstract Background Actinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial. Results In this study, about 34 r...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12934-024-02606-8 |
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| author | Mervat G. Hassan Gharieb S. El-Sayyad Mohamed O. Abdel-Monem Mohamed N. Malash Mona A. Kishk Mohamed E. El Awady Mohamed I. El-khonezy |
| author_facet | Mervat G. Hassan Gharieb S. El-Sayyad Mohamed O. Abdel-Monem Mohamed N. Malash Mona A. Kishk Mohamed E. El Awady Mohamed I. El-khonezy |
| author_sort | Mervat G. Hassan |
| collection | DOAJ |
| description | Abstract Background Actinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial. Results In this study, about 34 randomly selected Streptomyces isolates were discovered in soil, sediment, sea water, and other environments. Using a qualitative fast plate assay, they were tested for L-glutaminase production, and nine of them produced a significant amount of pink L-glutamine. Streptomyces sp. strain 5 M was identified by examining the 16S rRNA gene in the promising strain G8. A pH of 7.5, an incubation temperature of 40 °C, and the use of glucose and peptone as the carbon and nitrogen sources, respectively, produced the highest quantities of L-glutaminase. The molecular weight of the isolated L-glutaminase was estimated to be 52 kDa using SDS-PAGE analysis. At pH 7.5 and Temp., 40 °C, the isolated enzyme exhibited its highest levels of stability and activity. The isolated enzyme’s Km and Vmax values were 2.62 mM and 10.20 U/ml, respectively. Strong toxicity against HepG-2, HeLa, and MCF-7 was observed due to the anticancer properties of the isolated L-glutaminase. Conclusion Our findings include the discovery of Streptomyces sp. strain 5 M, which yields a free L-glutaminase and maybe a possible applicant for extra pharmacological investigation as an antineoplastic drug. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | Microbial Cell Factories |
| spelling | doaj-art-d0b6efb69da24fa59fc6cf8f48a7b0bb2025-01-05T12:50:47ZengBMCMicrobial Cell Factories1475-28592025-01-0124111710.1186/s12934-024-02606-8Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activityMervat G. Hassan0Gharieb S. El-Sayyad1Mohamed O. Abdel-Monem2Mohamed N. Malash3Mona A. Kishk4Mohamed E. El Awady5Mohamed I. El-khonezy6Department of Botany and Microbiology, Faculty of Science, Benha UniversityMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Badr University in Cairo (BUC)Department of Botany and Microbiology, Faculty of Science, Benha UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University (ACU)Department of Botany and Microbiology, Faculty of Science, Benha UniversityMicrobial Biotechnology Department, Biotechnology Research Institute, National Research CentreMolecular Biology Department, Biotechnology Research Institute, National Research CenterAbstract Background Actinomycetes are a well-known example of a microbiological origin that may generate a wide variety of chemical structures. As excellent cell factories, these sources are able to manufacture medicines, agrochemicals, and enzymes that are crucial. Results In this study, about 34 randomly selected Streptomyces isolates were discovered in soil, sediment, sea water, and other environments. Using a qualitative fast plate assay, they were tested for L-glutaminase production, and nine of them produced a significant amount of pink L-glutamine. Streptomyces sp. strain 5 M was identified by examining the 16S rRNA gene in the promising strain G8. A pH of 7.5, an incubation temperature of 40 °C, and the use of glucose and peptone as the carbon and nitrogen sources, respectively, produced the highest quantities of L-glutaminase. The molecular weight of the isolated L-glutaminase was estimated to be 52 kDa using SDS-PAGE analysis. At pH 7.5 and Temp., 40 °C, the isolated enzyme exhibited its highest levels of stability and activity. The isolated enzyme’s Km and Vmax values were 2.62 mM and 10.20 U/ml, respectively. Strong toxicity against HepG-2, HeLa, and MCF-7 was observed due to the anticancer properties of the isolated L-glutaminase. Conclusion Our findings include the discovery of Streptomyces sp. strain 5 M, which yields a free L-glutaminase and maybe a possible applicant for extra pharmacological investigation as an antineoplastic drug.https://doi.org/10.1186/s12934-024-02606-8L-glutaminaseCytotoxicityMarine waterStreptomyces sp.Strain 5 M. |
| spellingShingle | Mervat G. Hassan Gharieb S. El-Sayyad Mohamed O. Abdel-Monem Mohamed N. Malash Mona A. Kishk Mohamed E. El Awady Mohamed I. El-khonezy Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity Microbial Cell Factories L-glutaminase Cytotoxicity Marine water Streptomyces sp. Strain 5 M. |
| title | Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity |
| title_full | Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity |
| title_fullStr | Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity |
| title_full_unstemmed | Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity |
| title_short | Unravelling the outcome of L-glutaminase produced by Streptomyces sp. strain 5 M as an anti-neoplasm activity |
| title_sort | unravelling the outcome of l glutaminase produced by streptomyces sp strain 5 m as an anti neoplasm activity |
| topic | L-glutaminase Cytotoxicity Marine water Streptomyces sp. Strain 5 M. |
| url | https://doi.org/10.1186/s12934-024-02606-8 |
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