Chromosome-level genome assembly of stem borer Batocera rufomaculata using PacBio HiFi and Hi-C sequencing
Abstract Batocera rufomaculata (Cerambycidae: Lamiinae), a prominent representative of longhorned beetles, is a globally significant stem-boring pest, infesting over 50 species of deciduous trees. Despite its substantial ecological and economic impact, the genomic basis underlying its host adaptatio...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-07-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05463-1 |
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| Summary: | Abstract Batocera rufomaculata (Cerambycidae: Lamiinae), a prominent representative of longhorned beetles, is a globally significant stem-boring pest, infesting over 50 species of deciduous trees. Despite its substantial ecological and economic impact, the genomic basis underlying its host adaptation remain poorly understood. Here, we present a chromosome-level genome assembly of B. rufomaculata, constructed using a combination of Illumina, PacBio HiFi, and Hi-C sequencing data. The genome spans 338.08 Mb, with a scaffold N50 of 37.00 Mb, and is organized into 10 pseudo-chromosomes, including a chromosome X validated by genome collinearity and sequencing depth analyses. Repetitive elements constitute 27.89% of the genome, totaling 94.29 Mb. Out of 17,887 predicted genes, 12,729 were functionally annotated with at least one supporting evidence. The high-quality genome assembly and annotation were confirmed by multiple metrics, including genome size, reads mapping rate (>99.5%), BUSCO completeness (>97.1%), and collinearity analyses. This comprehensive genomic resource provides a foundation for investigating the ecological adaptation of B. rufomaculata and offers valuable insights into the genetic mechanisms that could inform pest management strategies. |
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| ISSN: | 2052-4463 |