Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features

This study investigated the effect of ultrasound-assisted enzymatic extraction (UAEE) on the extraction efficiency, antioxidant activity, and structural properties of jujube polysaccharide (JPS), with hot water extraction (HWE), ultrasound-assisted extraction (UAE), and enzymatic-assisted extraction...

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Main Authors: Yiting Guo, Shenao Nan, Chengcheng Qiu, Chenyu Song, Bengang Wu, Yanhua Tang, Lifang Cheng, Haile Ma
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Ultrasonics Sonochemistry
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Online Access:http://www.sciencedirect.com/science/article/pii/S1350417724003365
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author Yiting Guo
Shenao Nan
Chengcheng Qiu
Chenyu Song
Bengang Wu
Yanhua Tang
Lifang Cheng
Haile Ma
author_facet Yiting Guo
Shenao Nan
Chengcheng Qiu
Chenyu Song
Bengang Wu
Yanhua Tang
Lifang Cheng
Haile Ma
author_sort Yiting Guo
collection DOAJ
description This study investigated the effect of ultrasound-assisted enzymatic extraction (UAEE) on the extraction efficiency, antioxidant activity, and structural properties of jujube polysaccharide (JPS), with hot water extraction (HWE), ultrasound-assisted extraction (UAE), and enzymatic-assisted extraction (EAE) serving as controls. Optimal extraction conditions were determined through a multi-index weighted scoring method that comprehensively accounted for yield, duration, and antioxidant activity. Results demonstrated that the JPS yield obtained by UAEE at 22/33 kHz was 10.5 % to 16.3 % higher than those achieved by the other methods, significantly enhancing antioxidant activity. Monosaccharide composition analysis revealed that UAEE increased the content of key mono-sugars in JPS. Additionally, assessments of molecular weight distribution, zeta potential, and rheological properties showed that UAEE reduced the molecular weight and apparent viscosity of JPS, resulting in a looser structural configuration. These structural modifications were observed in scanning electron microscope (SEM) images, which revealed a filamentous branched morphology in JPS obtained through UAEE. Further observations using the atomic force microscope (AFM) indicated that the polysaccharide chains extracted by UAEE were shorter in length, lower in height, and free from aggregation.
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institution Kabale University
issn 1350-4177
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publishDate 2024-12-01
publisher Elsevier
record_format Article
series Ultrasonics Sonochemistry
spelling doaj-art-ce8bca5113924c169d83c2062c7e91bb2024-11-22T07:36:40ZengElsevierUltrasonics Sonochemistry1350-41772024-12-01111107088Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure featuresYiting Guo0Shenao Nan1Chengcheng Qiu2Chenyu Song3Bengang Wu4Yanhua Tang5Lifang Cheng6Haile Ma7School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China; Institute of Food Physical Processing, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China; Institute of Food Physical Processing, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China; Corresponding author at: School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.Regional Development of Governance Center, Yinhu Street, Fuyang District, Hangzhou, Zhejiang 311402, China; Corresponding author at: Regional Development of Governance Center, Yinhu Street, Fuyang District, Hangzhou, Zhejiang 311402, China.Hangzhou Amphenol JET Interconnect Technology Co., Ltd., No.29, FuTai Road, ZhongTai Industrial Zone, Hangzhou 311121, ChinaSchool of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China; Institute of Food Physical Processing, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, ChinaThis study investigated the effect of ultrasound-assisted enzymatic extraction (UAEE) on the extraction efficiency, antioxidant activity, and structural properties of jujube polysaccharide (JPS), with hot water extraction (HWE), ultrasound-assisted extraction (UAE), and enzymatic-assisted extraction (EAE) serving as controls. Optimal extraction conditions were determined through a multi-index weighted scoring method that comprehensively accounted for yield, duration, and antioxidant activity. Results demonstrated that the JPS yield obtained by UAEE at 22/33 kHz was 10.5 % to 16.3 % higher than those achieved by the other methods, significantly enhancing antioxidant activity. Monosaccharide composition analysis revealed that UAEE increased the content of key mono-sugars in JPS. Additionally, assessments of molecular weight distribution, zeta potential, and rheological properties showed that UAEE reduced the molecular weight and apparent viscosity of JPS, resulting in a looser structural configuration. These structural modifications were observed in scanning electron microscope (SEM) images, which revealed a filamentous branched morphology in JPS obtained through UAEE. Further observations using the atomic force microscope (AFM) indicated that the polysaccharide chains extracted by UAEE were shorter in length, lower in height, and free from aggregation.http://www.sciencedirect.com/science/article/pii/S1350417724003365Ultrasound-assisted enzymatic extractionJujube polysaccharideAntioxidant activityMulti-index weighted comprehensive scoring methodStructural properties
spellingShingle Yiting Guo
Shenao Nan
Chengcheng Qiu
Chenyu Song
Bengang Wu
Yanhua Tang
Lifang Cheng
Haile Ma
Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
Ultrasonics Sonochemistry
Ultrasound-assisted enzymatic extraction
Jujube polysaccharide
Antioxidant activity
Multi-index weighted comprehensive scoring method
Structural properties
title Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
title_full Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
title_fullStr Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
title_full_unstemmed Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
title_short Ultrasound-assisted enzymatic extraction of jujube (Ziziphus jujuba Mill.) polysaccharides: Extraction efficiency, antioxidant activity, and structure features
title_sort ultrasound assisted enzymatic extraction of jujube ziziphus jujuba mill polysaccharides extraction efficiency antioxidant activity and structure features
topic Ultrasound-assisted enzymatic extraction
Jujube polysaccharide
Antioxidant activity
Multi-index weighted comprehensive scoring method
Structural properties
url http://www.sciencedirect.com/science/article/pii/S1350417724003365
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AT chenyusong ultrasoundassistedenzymaticextractionofjujubeziziphusjujubamillpolysaccharidesextractionefficiencyantioxidantactivityandstructurefeatures
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AT yanhuatang ultrasoundassistedenzymaticextractionofjujubeziziphusjujubamillpolysaccharidesextractionefficiencyantioxidantactivityandstructurefeatures
AT lifangcheng ultrasoundassistedenzymaticextractionofjujubeziziphusjujubamillpolysaccharidesextractionefficiencyantioxidantactivityandstructurefeatures
AT hailema ultrasoundassistedenzymaticextractionofjujubeziziphusjujubamillpolysaccharidesextractionefficiencyantioxidantactivityandstructurefeatures