Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case
<b>Background</b>: Monomicrobial <i>Enterobacterales</i> necrotizing fasciitis is associated with exceedingly high mortality rates. Although effective antimicrobial therapy is an important part of treatment, the traditional microbiological diagnostic methods are not fast enou...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Diagnostics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-4418/15/1/58 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841549303719395328 |
|---|---|
| author | Zoltán Tóth Bence Balázs Walter P. Pfliegler Eszter Csoma László Majoros Dorka Szűcs Renátó Kovács |
| author_facet | Zoltán Tóth Bence Balázs Walter P. Pfliegler Eszter Csoma László Majoros Dorka Szűcs Renátó Kovács |
| author_sort | Zoltán Tóth |
| collection | DOAJ |
| description | <b>Background</b>: Monomicrobial <i>Enterobacterales</i> necrotizing fasciitis is associated with exceedingly high mortality rates. Although effective antimicrobial therapy is an important part of treatment, the traditional microbiological diagnostic methods are not fast enough to meaningfully influence early therapeutic decisions. <b>Methods</b>: Here, we report the application of the BioMérieux Biofire Filmarray Joint Infection Panel (BFJIP) for the rapid detection of the causative agent and susceptibility prediction in such a case. Aside from the BFJIP-based rapid diagnostic approach and culturing, the whole genome sequencing (WGS) of the causative agent was performed using Illumina MiSeq and Oxford Nanopore MinION platforms. <b>Results</b>: The BFJIP indicated the presence of <i>K. pneumoniae</i>, without KPC, VIM, IMP, NDM, OXA-48 carbapenemase genes, and CTX-M-type extended-spectrum beta-lactamases. Based on the WGS data, the isolate belonged to the K1-capsule-type ST23, harboured a pNTUH-2044-like plasmid, and was positive for all the virulence factors associated with this lineage. The conventional susceptibility results were also in accordance with the BFJIP results; the isolate lacked any of these acquired resistance mechanisms. <b>Conclusions</b>: Despite this being the first case of the successful identification of pathogenic bacteria in necrotising fasciitis using this assay, the BFJIP may become a useful tool for rapid identification of pathogens in necrotising fasciitis cases and guiding antimicrobial therapy for better clinical outcomes. |
| format | Article |
| id | doaj-art-ce5dc2c2764549e6a2b4a61c60408ec6 |
| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-ce5dc2c2764549e6a2b4a61c60408ec62025-01-10T13:16:35ZengMDPI AGDiagnostics2075-44182024-12-011515810.3390/diagnostics15010058Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis CaseZoltán Tóth0Bence Balázs1Walter P. Pfliegler2Eszter Csoma3László Majoros4Dorka Szűcs5Renátó Kovács6Medical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryMedical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Molecular Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen, 4032 Debrecen, HungaryMedical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryMedical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryMedical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryMedical Microbiology, Clinical Center, Faculty of Medicine, University of Debrecen, 4032 Debrecen, Hungary<b>Background</b>: Monomicrobial <i>Enterobacterales</i> necrotizing fasciitis is associated with exceedingly high mortality rates. Although effective antimicrobial therapy is an important part of treatment, the traditional microbiological diagnostic methods are not fast enough to meaningfully influence early therapeutic decisions. <b>Methods</b>: Here, we report the application of the BioMérieux Biofire Filmarray Joint Infection Panel (BFJIP) for the rapid detection of the causative agent and susceptibility prediction in such a case. Aside from the BFJIP-based rapid diagnostic approach and culturing, the whole genome sequencing (WGS) of the causative agent was performed using Illumina MiSeq and Oxford Nanopore MinION platforms. <b>Results</b>: The BFJIP indicated the presence of <i>K. pneumoniae</i>, without KPC, VIM, IMP, NDM, OXA-48 carbapenemase genes, and CTX-M-type extended-spectrum beta-lactamases. Based on the WGS data, the isolate belonged to the K1-capsule-type ST23, harboured a pNTUH-2044-like plasmid, and was positive for all the virulence factors associated with this lineage. The conventional susceptibility results were also in accordance with the BFJIP results; the isolate lacked any of these acquired resistance mechanisms. <b>Conclusions</b>: Despite this being the first case of the successful identification of pathogenic bacteria in necrotising fasciitis using this assay, the BFJIP may become a useful tool for rapid identification of pathogens in necrotising fasciitis cases and guiding antimicrobial therapy for better clinical outcomes.https://www.mdpi.com/2075-4418/15/1/58necrotising fasciitisfilmarrayhypervirulencediagnostic<i>Enterobacterales</i> |
| spellingShingle | Zoltán Tóth Bence Balázs Walter P. Pfliegler Eszter Csoma László Majoros Dorka Szűcs Renátó Kovács Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case Diagnostics necrotising fasciitis filmarray hypervirulence diagnostic <i>Enterobacterales</i> |
| title | Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case |
| title_full | Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case |
| title_fullStr | Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case |
| title_full_unstemmed | Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case |
| title_short | Application of Biofire Filmarray Joint Infection Panel for Rapid Identification of Aetiology in a Necrotizing Fasciitis Case |
| title_sort | application of biofire filmarray joint infection panel for rapid identification of aetiology in a necrotizing fasciitis case |
| topic | necrotising fasciitis filmarray hypervirulence diagnostic <i>Enterobacterales</i> |
| url | https://www.mdpi.com/2075-4418/15/1/58 |
| work_keys_str_mv | AT zoltantoth applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT bencebalazs applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT walterppfliegler applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT esztercsoma applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT laszlomajoros applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT dorkaszucs applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase AT renatokovacs applicationofbiofirefilmarrayjointinfectionpanelforrapididentificationofaetiologyinanecrotizingfasciitiscase |