Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli
Abstract Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-62404-7 |
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| author | Yannick Baffert Nathan Fraikin Yasmine Makhloufi Julie Baltenneck Marie-Eve Val Annick Dedieu-Berne Jonathan Degosserie Bogdan I. Iorga Pierre Bogaerts Erwan Gueguen Christian Lesterlin Sarah Bigot |
| author_facet | Yannick Baffert Nathan Fraikin Yasmine Makhloufi Julie Baltenneck Marie-Eve Val Annick Dedieu-Berne Jonathan Degosserie Bogdan I. Iorga Pierre Bogaerts Erwan Gueguen Christian Lesterlin Sarah Bigot |
| author_sort | Yannick Baffert |
| collection | DOAJ |
| description | Abstract Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in Enterobacteriaceae. Despite the problematic prevalence of pOXA-48, most research focus on epidemiology and genomics, leaving gaps in our understanding of the mechanisms behind its propagation. In this study, we use a transposon sequencing approach to identify genetic elements critical for plasmid stability, replication, and conjugative transfer. Our results identify a novel type I toxin-antitoxin system, uncharacterized essential maintenance factors, and components of the type IV secretion system and regulatory elements crucial for conjugation. This study advances our understanding of pOXA-48 biology, providing key insights into the genetic factors underlying its successful maintenance and spread in bacterial populations. |
| format | Article |
| id | doaj-art-cca6acf3cc1a47d2bc7bb3b255307ae9 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-cca6acf3cc1a47d2bc7bb3b255307ae92025-08-24T11:36:41ZengNature PortfolioNature Communications2041-17232025-08-0116111310.1038/s41467-025-62404-7Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coliYannick Baffert0Nathan Fraikin1Yasmine Makhloufi2Julie Baltenneck3Marie-Eve Val4Annick Dedieu-Berne5Jonathan Degosserie6Bogdan I. Iorga7Pierre Bogaerts8Erwan Gueguen9Christian Lesterlin10Sarah Bigot11Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Microbiologie Adaptation et Pathogénie (MAP), Université Lyon 1, INSA de Lyon, CNRS, UMR 5240Institut Pasteur, Université Paris Cité, CNRS UMR3525, Unité Plasticité du Génome Bactérien, Département Génomes et GénétiqueMicrobiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Namur Molecular Tech, UCLouvain, CHU UCL NamurUniversité Paris-Saclay, CNRS UPR 2301, Institut de Chimie des Substances NaturellesNational reference center for antimicrobial resistance in Gram negative, CHU UCL NamurMicrobiologie Adaptation et Pathogénie (MAP), Université Lyon 1, INSA de Lyon, CNRS, UMR 5240Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Microbiologie Moléculaire et Biochimie Structurale (MMSB), Université Lyon 1, CNRS, Inserm, UMR5086Abstract Conjugative plasmids are the main drivers of antibiotic resistance dissemination contributing to the emergence and extensive spread of multidrug resistance clinical bacterial pathogens. pOXA-48 plasmids, belonging to the IncL group, emerge as the primary vehicle for carbapenem resistance in Enterobacteriaceae. Despite the problematic prevalence of pOXA-48, most research focus on epidemiology and genomics, leaving gaps in our understanding of the mechanisms behind its propagation. In this study, we use a transposon sequencing approach to identify genetic elements critical for plasmid stability, replication, and conjugative transfer. Our results identify a novel type I toxin-antitoxin system, uncharacterized essential maintenance factors, and components of the type IV secretion system and regulatory elements crucial for conjugation. This study advances our understanding of pOXA-48 biology, providing key insights into the genetic factors underlying its successful maintenance and spread in bacterial populations.https://doi.org/10.1038/s41467-025-62404-7 |
| spellingShingle | Yannick Baffert Nathan Fraikin Yasmine Makhloufi Julie Baltenneck Marie-Eve Val Annick Dedieu-Berne Jonathan Degosserie Bogdan I. Iorga Pierre Bogaerts Erwan Gueguen Christian Lesterlin Sarah Bigot Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli Nature Communications |
| title | Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli |
| title_full | Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli |
| title_fullStr | Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli |
| title_full_unstemmed | Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli |
| title_short | Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli |
| title_sort | genetic determinants of poxa 48 plasmid maintenance and propagation in escherichia coli |
| url | https://doi.org/10.1038/s41467-025-62404-7 |
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