A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O

A VioA Variant Activates Antibiotic Streptogramins in the Heterologous Host <i>Streptomyces</i> sp. OUC20-O

Heterologous expression of the G231L variant of VioA into 16 strains of marine-derived <i>Streptomyces</i>, combined with bioactivity tracking, leads to the activation of seven antibiotic streptogramins (<b>1</b>–<b>7</b>) in <i>Streptomyces</i> sp. OU...

Full description

Saved in:

Bibliographic Details
Main Authors:	Jie Shan, Liangguang Yue, Luyao Xu, Runyi Wang, Qingzhou Meng, Jun Feng, Joon-Hee Lee, Ming Lu, Huayue Li
Format:	Article
Language:	English
Published:	MDPI AG 2025-05-01
Series:	Marine Drugs
Subjects:	heterologous expression marine-derived <i>Streptomyces</i> multidrug-resistant antibiotic streptogramin
Online Access:	https://www.mdpi.com/1660-3397/23/5/205
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Heterologous expression of the G231L variant of VioA into 16 strains of marine-derived <i>Streptomyces</i>, combined with bioactivity tracking, leads to the activation of seven antibiotic streptogramins (<b>1</b>–<b>7</b>) in <i>Streptomyces</i> sp. OUC20-O. Among these, compound <b>1</b>, named linstreptogramin, is a new compound with an unusual linear streptogramin skeleton. The planar structure and stereochemistry of compound <b>1</b> were established based on extensive MS and NMR spectroscopic analyses, together with ECD calculations. In the antibacterial activity evaluation, compounds <b>1</b>–<b>4</b> showed significant growth inhibition against the multidrug-resistant <i>Enterococcus faecium</i> CCARM 5203 with MIC values of 0.2–1.6 µg/mL, which are comparable to the positive control vancomycin.
ISSN:	1660-3397