microRNA-320d通过PBX3抑制子宫内膜癌JEC细胞上皮-间质转化功能
【目的】研究microRNA-320d（miR-320d）对子宫内膜癌JEC细胞上皮-间质转化功能的影响及其相关机制。【方 法】 JEC子宫内膜癌细胞株分别转染miR-320d mimics和negative control mimic，分别作为M320d、NCM组，并设立未转染对 照control组，采用RT-PCR法检测各组细胞miR-320d含量。Transwell实验检测3组细胞迁移能力和侵袭能力。Western- blot法检测3组细胞α-Catenin和PBX3表达水平，及PBX3过表达对miR-320d抑制EMT的拮抗作用。双荧光素酶实验检测 miR-320d与PBX3的...
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| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Sun Yat-sen University
2017-01-01
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| Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
| Subjects: | |
| Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43567995/ |
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