Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment
Abstract Proteomic communications in neighboring microenvironments during early organ development is a dynamic process that continuously reshapes human embryonic stem cells (hESCs) developmental fate. Such dynamic proteomic alteration in the microenvironment consists of both freely secreted proteome...
Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-01-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202406509 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841543170371878912 |
|---|---|
| author | Haoni Yan Aynur Abdulla Aiting Wang Shuyu Ding Manlin Zhang Yizhi Zhang Tsz Yui Zhuang Leqi Wu Yan Wang Rongrong Ren Lai Jiang Xianting Ding |
| author_facet | Haoni Yan Aynur Abdulla Aiting Wang Shuyu Ding Manlin Zhang Yizhi Zhang Tsz Yui Zhuang Leqi Wu Yan Wang Rongrong Ren Lai Jiang Xianting Ding |
| author_sort | Haoni Yan |
| collection | DOAJ |
| description | Abstract Proteomic communications in neighboring microenvironments during early organ development is a dynamic process that continuously reshapes human embryonic stem cells (hESCs) developmental fate. Such dynamic proteomic alteration in the microenvironment consists of both freely secreted proteome and exosome‐encapsulated proteome. Simultaneous monitoring of the time‐lapse shift of both proteomes with live organoids remains technically challenging. Here, a continuous organoid secretion/encapsulation proteome tandem LC‐MS/MS (COSEP‐LCM) is introduced, which permits time‐lapse monitoring of proteomic alterations both in free secretion form and in exosome encapsulated form at live organoids’ microenvironment. Continuous growth of human cerebral organoids (COs) and free‐secretion/exosome‐encapsulation proteomics acquisition with COSEP‐LCM for 60 days is demonstrated. SERPINF1, F5, and EFNB1 are initially enriched inside exosomes as encapsulated excretion and then gradually enriched outside exosomes as freely secreted excretion, while C3 is initially enriched outside exosomes as freely secreted excretion and gradually enriched inside exosomes as encapsulated excretion. Such dynamic excretion pattern paradigm shift may imply critical developmental strategy evolution during early human cerebral development. COSEP‐LCM offers a platform technique for continuous inside/outside exosome proteomics co‐analysis in live organoids’ microenvironment. |
| format | Article |
| id | doaj-art-cbb1a7be81d948c1be25cb63cfd199b5 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-cbb1a7be81d948c1be25cb63cfd199b52025-01-13T15:29:43ZengWileyAdvanced Science2198-38442025-01-01122n/an/a10.1002/advs.202406509Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ MicroenvironmentHaoni Yan0Aynur Abdulla1Aiting Wang2Shuyu Ding3Manlin Zhang4Yizhi Zhang5Tsz Yui Zhuang6Leqi Wu7Yan Wang8Rongrong Ren9Lai Jiang10Xianting Ding11Department of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaState Key Laboratory of Oncogenes and Related Genes Institute for Personalized Medicine Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaState Key Laboratory of Oncogenes and Related Genes Institute for Personalized Medicine Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaState Key Laboratory of Oncogenes and Related Genes Institute for Personalized Medicine Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaState Key Laboratory of Oncogenes and Related Genes Institute for Personalized Medicine Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital Shanghai Jiaotong University School of Medicine Shanghai 200092 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaDepartment of Anesthesiology and Surgical Intensive Care Unit Xinhua Hospital School of Medicine and School of Biomedical Engineering Shanghai Jiao Tong University Shanghai 200030 P. R. ChinaAbstract Proteomic communications in neighboring microenvironments during early organ development is a dynamic process that continuously reshapes human embryonic stem cells (hESCs) developmental fate. Such dynamic proteomic alteration in the microenvironment consists of both freely secreted proteome and exosome‐encapsulated proteome. Simultaneous monitoring of the time‐lapse shift of both proteomes with live organoids remains technically challenging. Here, a continuous organoid secretion/encapsulation proteome tandem LC‐MS/MS (COSEP‐LCM) is introduced, which permits time‐lapse monitoring of proteomic alterations both in free secretion form and in exosome encapsulated form at live organoids’ microenvironment. Continuous growth of human cerebral organoids (COs) and free‐secretion/exosome‐encapsulation proteomics acquisition with COSEP‐LCM for 60 days is demonstrated. SERPINF1, F5, and EFNB1 are initially enriched inside exosomes as encapsulated excretion and then gradually enriched outside exosomes as freely secreted excretion, while C3 is initially enriched outside exosomes as freely secreted excretion and gradually enriched inside exosomes as encapsulated excretion. Such dynamic excretion pattern paradigm shift may imply critical developmental strategy evolution during early human cerebral development. COSEP‐LCM offers a platform technique for continuous inside/outside exosome proteomics co‐analysis in live organoids’ microenvironment.https://doi.org/10.1002/advs.202406509cerebral organoidsexosomesmicroenvironmentmicrofluidic platformproteomics |
| spellingShingle | Haoni Yan Aynur Abdulla Aiting Wang Shuyu Ding Manlin Zhang Yizhi Zhang Tsz Yui Zhuang Leqi Wu Yan Wang Rongrong Ren Lai Jiang Xianting Ding Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment Advanced Science cerebral organoids exosomes microenvironment microfluidic platform proteomics |
| title | Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment |
| title_full | Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment |
| title_fullStr | Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment |
| title_full_unstemmed | Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment |
| title_short | Time‐Lapse Acquisition of Both Freely Secreted Proteome and Exosome Encapsulated Proteome in Live Organoids’ Microenvironment |
| title_sort | time lapse acquisition of both freely secreted proteome and exosome encapsulated proteome in live organoids microenvironment |
| topic | cerebral organoids exosomes microenvironment microfluidic platform proteomics |
| url | https://doi.org/10.1002/advs.202406509 |
| work_keys_str_mv | AT haoniyan timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT aynurabdulla timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT aitingwang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT shuyuding timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT manlinzhang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT yizhizhang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT tszyuizhuang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT leqiwu timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT yanwang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT rongrongren timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT laijiang timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment AT xiantingding timelapseacquisitionofbothfreelysecretedproteomeandexosomeencapsulatedproteomeinliveorganoidsmicroenvironment |