Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines
Avian pathogen Escherichia coli (APEC) poses a significant threat to poultry farming, causing colibacillosis in various hosts, including chickens, ducks, geese, and pigeons. This study aimed to investigate the molecular epidemiology of APEC in Jiangxi Province, China, to inform prevention and contro...
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000033 |
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| author | Jia Tan Ying-Xin Wang Zhao-Feng Kang Jiang-Nan Huang Hai-Qin Li Cheng-Cheng Wu Na Li Fan-Fan Zhang Mei-Fang Tan |
| author_facet | Jia Tan Ying-Xin Wang Zhao-Feng Kang Jiang-Nan Huang Hai-Qin Li Cheng-Cheng Wu Na Li Fan-Fan Zhang Mei-Fang Tan |
| author_sort | Jia Tan |
| collection | DOAJ |
| description | Avian pathogen Escherichia coli (APEC) poses a significant threat to poultry farming, causing colibacillosis in various hosts, including chickens, ducks, geese, and pigeons. This study aimed to investigate the molecular epidemiology of APEC in Jiangxi Province, China, to inform prevention and control strategies. Between 2020 and 2024, 186 APEC strains were isolated and identified, with 88.7 % from liver samples and 5.4 % from brain tissues. Whole-genome sequencing and analysis revealed that serotypes O145, O78, O8, and O111 comprised 32.9 %, 9.3 %, 7.9 %, and 4.3 % of the isolates, respectively. The dominant H serotypes included H21 (23.8 %), H9 (22.7 %), and H4 (18.2 %), with ST23 being the most prevalent sequence type at 24.2 %, followed by ST117 (10.1 %) and ST2505 (7.3 %). Virulence-associated genes in the strains O145, O78, O8, and O111 were further analyzed for quantity and pattern. Eight strains were selected for in vivo pathogenicity testing in ducks, four highly pathogenic strains were then identified, which were used to develop inactivated vaccines with the novel aqueous adjuvant MONTANIDE™ GEL P PR and the traditional adjuvant alumVax hydroxide. Compared with existing vaccines, those formulated in this study showed superior protection, with the MONTANIDE™ GEL P PR adjuvant achieving the highest protection rate (90.0 %, 36/40) and lower lesion scores. This research enhances the understanding of APEC epidemiology and offers promising strategies for effective vaccine development against prevalent APEC. |
| format | Article |
| id | doaj-art-c9ba03a036564333a34fab51c31b3814 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-c9ba03a036564333a34fab51c31b38142025-01-06T04:08:30ZengElsevierPoultry Science0032-57912025-02-011042104766Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccinesJia Tan0Ying-Xin Wang1Zhao-Feng Kang2Jiang-Nan Huang3Hai-Qin Li4Cheng-Cheng Wu5Na Li6Fan-Fan Zhang7Mei-Fang Tan8Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaAnimal Disease Prevention and Control Center, Ezhou 436000, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR ChinaInstitute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China; Jiangxi Provincial Key Laboratory of Green and Healthy Breeding of Livestock and Poultry, Nanchang 330200, PR China; Corresponding author at: Institute of Animal Husbandry and Veterinary Science, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, PR China.Avian pathogen Escherichia coli (APEC) poses a significant threat to poultry farming, causing colibacillosis in various hosts, including chickens, ducks, geese, and pigeons. This study aimed to investigate the molecular epidemiology of APEC in Jiangxi Province, China, to inform prevention and control strategies. Between 2020 and 2024, 186 APEC strains were isolated and identified, with 88.7 % from liver samples and 5.4 % from brain tissues. Whole-genome sequencing and analysis revealed that serotypes O145, O78, O8, and O111 comprised 32.9 %, 9.3 %, 7.9 %, and 4.3 % of the isolates, respectively. The dominant H serotypes included H21 (23.8 %), H9 (22.7 %), and H4 (18.2 %), with ST23 being the most prevalent sequence type at 24.2 %, followed by ST117 (10.1 %) and ST2505 (7.3 %). Virulence-associated genes in the strains O145, O78, O8, and O111 were further analyzed for quantity and pattern. Eight strains were selected for in vivo pathogenicity testing in ducks, four highly pathogenic strains were then identified, which were used to develop inactivated vaccines with the novel aqueous adjuvant MONTANIDE™ GEL P PR and the traditional adjuvant alumVax hydroxide. Compared with existing vaccines, those formulated in this study showed superior protection, with the MONTANIDE™ GEL P PR adjuvant achieving the highest protection rate (90.0 %, 36/40) and lower lesion scores. This research enhances the understanding of APEC epidemiology and offers promising strategies for effective vaccine development against prevalent APEC.http://www.sciencedirect.com/science/article/pii/S0032579125000033Avian pathogenic Escherichia coliWhole-genome sequencingVirulence-associated geneSerotypeVaccine |
| spellingShingle | Jia Tan Ying-Xin Wang Zhao-Feng Kang Jiang-Nan Huang Hai-Qin Li Cheng-Cheng Wu Na Li Fan-Fan Zhang Mei-Fang Tan Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines Poultry Science Avian pathogenic Escherichia coli Whole-genome sequencing Virulence-associated gene Serotype Vaccine |
| title | Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines |
| title_full | Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines |
| title_fullStr | Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines |
| title_full_unstemmed | Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines |
| title_short | Molecular characterization of avian pathogenic Escherichia coli strains in Jiangxi Province, China, and development of polyvalent inactivated vaccines |
| title_sort | molecular characterization of avian pathogenic escherichia coli strains in jiangxi province china and development of polyvalent inactivated vaccines |
| topic | Avian pathogenic Escherichia coli Whole-genome sequencing Virulence-associated gene Serotype Vaccine |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000033 |
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