Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum
Abstract Optimized production of Aspergillus niger ATCC 26011 endo-β-mannanase (ManAn) on copra meal resulted in 2.46-fold increase (10,028 U/gds). Purified ManAn (47 kDa) showed high affinity towards guar gum (GG) as compared to konjac gum and locust bean gum with Km 2.67, 3.25 and 4.07 mg/mL, resp...
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Nature Portfolio
2024-06-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-63803-4 |
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| author | Suresh Nath Naveen Kango |
| author_facet | Suresh Nath Naveen Kango |
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| description | Abstract Optimized production of Aspergillus niger ATCC 26011 endo-β-mannanase (ManAn) on copra meal resulted in 2.46-fold increase (10,028 U/gds). Purified ManAn (47 kDa) showed high affinity towards guar gum (GG) as compared to konjac gum and locust bean gum with Km 2.67, 3.25 and 4.07 mg/mL, respectively. ManAn efficiently hydrolyzed GG and liberated mannooligosaccharides (MOS). Changes occurring in the rheological and compositional aspects of GG studied using Differential scanning calorimetry (DSC), Thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) revealed increased thermal stability and crystallinity of the partially hydrolyzed guar gum (PHGG). Parametric optimization of the time and temperature dependent hydrolysis of GG (1% w/v) with 100 U/mL of ManAn at 60 °C and pH: 5.0 resulted in 12.126 mg/mL of mannotetraose (M4) in 5 min. Enhanced growth of probiotics Lactobacilli and production of short chain fatty acids (SCFA) that inhibited enteropathogens, confirmed the prebiotic potential of PHGG and M4. |
| format | Article |
| id | doaj-art-c972b6c6cc1241ff9cf776a5a0e9512c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-c972b6c6cc1241ff9cf776a5a0e9512c2025-01-12T12:24:59ZengNature PortfolioScientific Reports2045-23222024-06-0114111610.1038/s41598-024-63803-4Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gumSuresh Nath0Naveen Kango1Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University)Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University)Abstract Optimized production of Aspergillus niger ATCC 26011 endo-β-mannanase (ManAn) on copra meal resulted in 2.46-fold increase (10,028 U/gds). Purified ManAn (47 kDa) showed high affinity towards guar gum (GG) as compared to konjac gum and locust bean gum with Km 2.67, 3.25 and 4.07 mg/mL, respectively. ManAn efficiently hydrolyzed GG and liberated mannooligosaccharides (MOS). Changes occurring in the rheological and compositional aspects of GG studied using Differential scanning calorimetry (DSC), Thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) revealed increased thermal stability and crystallinity of the partially hydrolyzed guar gum (PHGG). Parametric optimization of the time and temperature dependent hydrolysis of GG (1% w/v) with 100 U/mL of ManAn at 60 °C and pH: 5.0 resulted in 12.126 mg/mL of mannotetraose (M4) in 5 min. Enhanced growth of probiotics Lactobacilli and production of short chain fatty acids (SCFA) that inhibited enteropathogens, confirmed the prebiotic potential of PHGG and M4.https://doi.org/10.1038/s41598-024-63803-4β-mannanaseGuar gumPartially hydrolyzed guar gumA. nigerPrebioticsProbiotics |
| spellingShingle | Suresh Nath Naveen Kango Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum Scientific Reports β-mannanase Guar gum Partially hydrolyzed guar gum A. niger Prebiotics Probiotics |
| title | Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| title_full | Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| title_fullStr | Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| title_full_unstemmed | Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| title_short | Optimized production and characterization of endo-β-mannanase by Aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| title_sort | optimized production and characterization of endo β mannanase by aspergillus niger for generation of prebiotic mannooligosaccharides from guar gum |
| topic | β-mannanase Guar gum Partially hydrolyzed guar gum A. niger Prebiotics Probiotics |
| url | https://doi.org/10.1038/s41598-024-63803-4 |
| work_keys_str_mv | AT sureshnath optimizedproductionandcharacterizationofendobmannanasebyaspergillusnigerforgenerationofprebioticmannooligosaccharidesfromguargum AT naveenkango optimizedproductionandcharacterizationofendobmannanasebyaspergillusnigerforgenerationofprebioticmannooligosaccharidesfromguargum |