Development of an in-house dual RT-qPCR assay for detecting SFTSV and Hantaan virus simultaneously

Development of an in-house dual RT-qPCR assay for detecting SFTSV and Hantaan virus simultaneously

Given the overlapping endemic regions and clinical similarities between severe fever with thrombocytopenia syndrome (SFTS) and hemorrhagic fever with renal syndrome (HFRS), we developed a dual real‐time fluorescence‐based reverse transcription quantitative polymerase chain reaction (RT-qPCR) method....

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Bibliographic Details
Main Authors: Xiaoyu Xue, Youde Liu, Chuan Song, Tingting Liu, Zishuai Liu, Wenjing Niu, Zhouling Jiang, Yanli Xu, Yuanyuan Zhang, Ling Lin, Zhihai Chen
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Biosafety and Health
Subjects:
Severe fever with thrombocytopenia syndrome virus (SFTSV)
Hantaan virus
Hemorrhagic fever with renal syndrome (HFRS)
Severe fever with thrombocytopenia syndrome (SFTS)
Differential diagnosis
Dual real time reverse transcription quantitative polymerase chain reaction (RT-qPCR)
Online Access:http://www.sciencedirect.com/science/article/pii/S2590053625000436
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