Impact of sample multiplexing on detection of bacteria and antimicrobial resistance genes in pig microbiomes using long-read sequencing

Impact of sample multiplexing on detection of bacteria and antimicrobial resistance genes in pig microbiomes using long-read sequencing

The effects of sample multiplexing on the detection sensitivity of antimicrobial resistance genes (ARGs) and pathogenic bacteria in metagenomic sequencing remain underexplored in newer sequencing technologies such as Oxford Nanopore Technologies (ONT), despite its critical importance for surveillanc...

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Bibliographic Details
Main Authors: Mirena Ivanova, Frank M. Aarestrup, Saria Otani
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Microbiology
Subjects:
microbiome
long read sequencing
AMR
bacteria
multiplexing
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1597804/full
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