Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation
Background. The epidemic situation regarding measles both in Russia and in the world makes it extremely urgent to introduce highly sensitive molecular methods, such as polymerase chain reaction, into diagnostics, in addition to existing serological ones. This paper describes the experience of using...
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| Format: | Article |
| Language: | Russian |
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Open Systems Publication
2024-11-01
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| Series: | Лечащий Врач |
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| Online Access: | https://journal.lvrach.ru/jour/article/view/1313 |
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| author | T. L. Zamotaeva E. A. Cherkashin M. A. Ninalalov Zh. B. Ponezheva V. G. Akimkin |
| author_facet | T. L. Zamotaeva E. A. Cherkashin M. A. Ninalalov Zh. B. Ponezheva V. G. Akimkin |
| author_sort | T. L. Zamotaeva |
| collection | DOAJ |
| description | Background. The epidemic situation regarding measles both in Russia and in the world makes it extremely urgent to introduce highly sensitive molecular methods, such as polymerase chain reaction, into diagnostics, in addition to existing serological ones. This paper describes the experience of using the polymerase chain reaction method for diagnosing measles in the Russian Federation using the example of the Republic of Dagestan.Objective. Early detection of measles virus using a highly sensitive polymerase chain reaction method in various types of biomaterial.Materials and methods. The study was carried out using the polymerase chain reaction method on biomaterial samples from 208 patients diagnosed with measles and 30 apparently healthy volunteers in the control group. The diagnosis of measles was established based on a combination of clinical manifestations and confirmed by the results of laboratory testing with the detection of IgM class antibodies using enzyme linked immunosorbent assay.Results. Patients admitted to the hospital before 4 days from the onset of the rash were found to be positive by polymerase chain reaction testing for measles virus RNA. The viral load in urine samples and swabs from the nasopharynx and oropharynx was 103-109 GE/ml, of which in 67.5% of cases it was 106-109 GE/ml; while a much smaller amount of viral RNA was detected in the blood serum – 103-105 GE/ml.Conclusion. The possibility of early detection of measles using the polymerase chain reaction method has been shown, which will improve the process of differential diagnosis of diseases that have a similar clinical picture when introducing polymerase chain reaction tests for measles into practical healthcare. This approach involves testing not only sick people, but also contact persons. |
| format | Article |
| id | doaj-art-c7b22275e45d4572aaa2c74dfb02d8b7 |
| institution | Kabale University |
| issn | 1560-5175 2687-1181 |
| language | Russian |
| publishDate | 2024-11-01 |
| publisher | Open Systems Publication |
| record_format | Article |
| series | Лечащий Врач |
| spelling | doaj-art-c7b22275e45d4572aaa2c74dfb02d8b72025-08-20T03:57:51ZrusOpen Systems PublicationЛечащий Врач1560-51752687-11812024-11-01011656910.51793/OS.2024.27.11.0111300Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian FederationT. L. Zamotaeva0E. A. Cherkashin1M. A. Ninalalov2Zh. B. Ponezheva3V. G. Akimkin4Central Research Institute of Epidemiology of RospotrebnadzorCentral Research Institute of Epidemiology of RospotrebnadzorRepublican Center for Infectious Diseases, Prevention and Control of AIDS named after S. M. MagomedovCentral Research Institute of Epidemiology of RospotrebnadzorCentral Research Institute of Epidemiology of RospotrebnadzorBackground. The epidemic situation regarding measles both in Russia and in the world makes it extremely urgent to introduce highly sensitive molecular methods, such as polymerase chain reaction, into diagnostics, in addition to existing serological ones. This paper describes the experience of using the polymerase chain reaction method for diagnosing measles in the Russian Federation using the example of the Republic of Dagestan.Objective. Early detection of measles virus using a highly sensitive polymerase chain reaction method in various types of biomaterial.Materials and methods. The study was carried out using the polymerase chain reaction method on biomaterial samples from 208 patients diagnosed with measles and 30 apparently healthy volunteers in the control group. The diagnosis of measles was established based on a combination of clinical manifestations and confirmed by the results of laboratory testing with the detection of IgM class antibodies using enzyme linked immunosorbent assay.Results. Patients admitted to the hospital before 4 days from the onset of the rash were found to be positive by polymerase chain reaction testing for measles virus RNA. The viral load in urine samples and swabs from the nasopharynx and oropharynx was 103-109 GE/ml, of which in 67.5% of cases it was 106-109 GE/ml; while a much smaller amount of viral RNA was detected in the blood serum – 103-105 GE/ml.Conclusion. The possibility of early detection of measles using the polymerase chain reaction method has been shown, which will improve the process of differential diagnosis of diseases that have a similar clinical picture when introducing polymerase chain reaction tests for measles into practical healthcare. This approach involves testing not only sick people, but also contact persons.https://journal.lvrach.ru/jour/article/view/1313measlesdiagnosispolymerase chain reactionenzyme linked immunosorbent assay |
| spellingShingle | T. L. Zamotaeva E. A. Cherkashin M. A. Ninalalov Zh. B. Ponezheva V. G. Akimkin Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation Лечащий Врач measles diagnosis polymerase chain reaction enzyme linked immunosorbent assay |
| title | Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation |
| title_full | Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation |
| title_fullStr | Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation |
| title_full_unstemmed | Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation |
| title_short | Experience in using the polymerase chain reaction method for diagnosis of measles in the Russian Federation |
| title_sort | experience in using the polymerase chain reaction method for diagnosis of measles in the russian federation |
| topic | measles diagnosis polymerase chain reaction enzyme linked immunosorbent assay |
| url | https://journal.lvrach.ru/jour/article/view/1313 |
| work_keys_str_mv | AT tlzamotaeva experienceinusingthepolymerasechainreactionmethodfordiagnosisofmeaslesintherussianfederation AT eacherkashin experienceinusingthepolymerasechainreactionmethodfordiagnosisofmeaslesintherussianfederation AT maninalalov experienceinusingthepolymerasechainreactionmethodfordiagnosisofmeaslesintherussianfederation AT zhbponezheva experienceinusingthepolymerasechainreactionmethodfordiagnosisofmeaslesintherussianfederation AT vgakimkin experienceinusingthepolymerasechainreactionmethodfordiagnosisofmeaslesintherussianfederation |