Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle
Decoctions of stem barks from <i>Aucoumea klaineana</i>, <i>Canarium schweinfurthii</i>, <i>Pentadesma butyracea</i> and <i>Scorodophloeus zenkeri</i> are used against affections of irritable bowel syndrome in Gabonese traditional medicine. In the pres...
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2025-07-01
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| author | Ericka Lorleil Mayindza Ekaghba Olivier Perruchon Patrice Lerouge Line Edwige Mengome |
| author_facet | Ericka Lorleil Mayindza Ekaghba Olivier Perruchon Patrice Lerouge Line Edwige Mengome |
| author_sort | Ericka Lorleil Mayindza Ekaghba |
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| description | Decoctions of stem barks from <i>Aucoumea klaineana</i>, <i>Canarium schweinfurthii</i>, <i>Pentadesma butyracea</i> and <i>Scorodophloeus zenkeri</i> are used against affections of irritable bowel syndrome in Gabonese traditional medicine. In the present study, we aim to determine whether the bark polysaccharides may contribute to the activity of these plants against the symptoms of gastrointestinal disorders. To this end, we investigated the structure and the pharmacological activity of polysaccharides extracted from their stem barks. The pectic and hemicellulose polysaccharides were isolated, and their sugar compositions were determined by gas chromatography. In addition, analysis by MALDI-TOF mass spectrometry of oligosaccharides released after digestion with an endo-xylanase indicated that glucuronoarabinoxylans are the main hemicellulose of stem barks. We then evaluated the influence of the polysaccharide fractions on the spontaneous contractile activity of rat ileal smooth muscle and the cholinergic system. Spasmolytic activity of pectic fractions from all stem barks, as well as lemon polygalacturonic acid, were observed, indicating that these extracts exhibit a myorelaxant activity. In contrast, the bark hemicellulose fractions, as well as commercially available beechwood glucuronoxylan and wheat arabinoxylan, were demonstrated to be able to increase the basal contractile activity of smooth muscle. These data show that, beyond physicochemical effects affecting the bowel water content, plant polysaccharides have also an impact on the spontaneous smooth muscle contractility, the main mechanism involved in the pathophysiology of gastrointestinal disorders. |
| format | Article |
| id | doaj-art-c681e74160124e02b2bda97fb65b68a6 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-c681e74160124e02b2bda97fb65b68a62025-08-20T04:00:53ZengMDPI AGMolecules1420-30492025-07-013015315610.3390/molecules30153156Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth MuscleEricka Lorleil Mayindza Ekaghba0Olivier Perruchon1Patrice Lerouge2Line Edwige Mengome3Institut de Pharmacopée et Médecines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, GabonUniversité de Rouen Normandie (UNIROUEN), Normandie University, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT Bestim, F-76000 Rouen, FranceUniversité de Rouen Normandie (UNIROUEN), Normandie University, GlycoMEV UR 4358, SFR Normandie Végétal FED 4277, Innovation Chimie Carnot, IRIB, GDR CNRS Chemobiologie, RMT Bestim, F-76000 Rouen, FranceInstitut de Pharmacopée et Médecines Traditionnelles (IPHAMETRA), Centre National de la Recherche Scientifique et Technique (CENAREST), Libreville BP 12 141, GabonDecoctions of stem barks from <i>Aucoumea klaineana</i>, <i>Canarium schweinfurthii</i>, <i>Pentadesma butyracea</i> and <i>Scorodophloeus zenkeri</i> are used against affections of irritable bowel syndrome in Gabonese traditional medicine. In the present study, we aim to determine whether the bark polysaccharides may contribute to the activity of these plants against the symptoms of gastrointestinal disorders. To this end, we investigated the structure and the pharmacological activity of polysaccharides extracted from their stem barks. The pectic and hemicellulose polysaccharides were isolated, and their sugar compositions were determined by gas chromatography. In addition, analysis by MALDI-TOF mass spectrometry of oligosaccharides released after digestion with an endo-xylanase indicated that glucuronoarabinoxylans are the main hemicellulose of stem barks. We then evaluated the influence of the polysaccharide fractions on the spontaneous contractile activity of rat ileal smooth muscle and the cholinergic system. Spasmolytic activity of pectic fractions from all stem barks, as well as lemon polygalacturonic acid, were observed, indicating that these extracts exhibit a myorelaxant activity. In contrast, the bark hemicellulose fractions, as well as commercially available beechwood glucuronoxylan and wheat arabinoxylan, were demonstrated to be able to increase the basal contractile activity of smooth muscle. These data show that, beyond physicochemical effects affecting the bowel water content, plant polysaccharides have also an impact on the spontaneous smooth muscle contractility, the main mechanism involved in the pathophysiology of gastrointestinal disorders.https://www.mdpi.com/1420-3049/30/15/3156pectinhemicelluloseglucuronoarabinoxylanspasmolytic activityspasmogenic activity |
| spellingShingle | Ericka Lorleil Mayindza Ekaghba Olivier Perruchon Patrice Lerouge Line Edwige Mengome Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle Molecules pectin hemicellulose glucuronoarabinoxylan spasmolytic activity spasmogenic activity |
| title | Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle |
| title_full | Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle |
| title_fullStr | Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle |
| title_full_unstemmed | Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle |
| title_short | Effects of Polysaccharides Extracted from Stem Barks on the Spontaneous Contractile Activity of the Ileal Smooth Muscle |
| title_sort | effects of polysaccharides extracted from stem barks on the spontaneous contractile activity of the ileal smooth muscle |
| topic | pectin hemicellulose glucuronoarabinoxylan spasmolytic activity spasmogenic activity |
| url | https://www.mdpi.com/1420-3049/30/15/3156 |
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