Transcriptomic Analysis of Cell Stress Response in <i>Wickerhamomyces anomalus</i> H4 Under Octanoic Acid Stress
The purified yeast strain H4, identified as <i>W. anomalus</i> through morphological, genetic, and phylogenetic analyses, was characterized and compared to a commercial <i>Saccharomyces cerevisiae</i> strain X16. <i>W. anomalus</i> H4 exhibited distinct morphologi...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/10/11/563 |
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| Summary: | The purified yeast strain H4, identified as <i>W. anomalus</i> through morphological, genetic, and phylogenetic analyses, was characterized and compared to a commercial <i>Saccharomyces cerevisiae</i> strain X16. <i>W. anomalus</i> H4 exhibited distinct morphological features. It demonstrated notable tolerance to 11% ethanol, 220 g/L glucose, and 200 mg/L octanoic acid, similar to X16, except for having a lower tolerance to SO<sub>2</sub>. Survival analysis under various stress conditions revealed that ethanol and octanoic acid had the most detrimental effects, with 56% cell mortality at 13% ethanol and 400 mg/L octanoic acid. Transcriptomic analysis under octanoic acid stress showed that at 200 mg/L, 3369 differentially expressed genes (DEGs) were induced, with 1609 being upregulated and 1760 downregulated, indicating broad transcriptional reprogramming. At 400 mg/L, only 130 DEGs were detected, suggesting a more limited response. KEGG pathway analysis indicated that most DEGs at 200 mg/L were associated with the “ribosome” and “proteasome” pathways, reflecting disruptions in protein synthesis and turnover. At 400 mg/L, the DEGs were primarily related to “DNA replication” and “pyruvate metabolism”. These findings highlight the adaptive mechanisms of <i>W. anomalus</i> H4 to environmental stresses, particularly octanoic acid, and its potential for use in brewing and fermentation processes. |
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| ISSN: | 2311-5637 |