Functional characterization and unraveling the structural determinants of novel steroid hydroxylase CYP154C7 from Streptomyces sp. PAMC26508

Functional characterization and unraveling the structural determinants of novel steroid hydroxylase CYP154C7 from Streptomyces sp. PAMC26508

This study characterized cytochrome P450 enzyme CYP154C7 from Streptomyces sp. PAMC26508, emphasizing its capability to hydroxylate steroids, especially at the 16α-position. The enzymatic assay of CYP154C7 demonstrated effective conversion across a pH range of 7.2–7.6, with optimal activity at 30 °C...

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Bibliographic Details
Main Authors: Prakash Paudel, Kamal Prasad Regmi, Ki-Hwa Kim, Jun Hyuck Lee, Tae-Jin Oh
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Heliyon
Subjects:
Steroid hydroxylases
Cytochrome P450
Streptomyces
Molecular docking
Biocatalyst
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024158082
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