Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin
<b>Aim: </b>The purpose was to investigate the effects of various dosages of thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced hepatotoxicity in rats from a pathological standpoint.<p> <b>Materials and Methods:</b> Eighty male Wistar Albino rats were...
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Selcuk University Press
2022-09-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1398 |
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| author | Mehmet Burak Ates Ozgur Ozdemir Ali Erdem Ozturk Mustafa Numan Bucak Aysegul Bulut |
| author_facet | Mehmet Burak Ates Ozgur Ozdemir Ali Erdem Ozturk Mustafa Numan Bucak Aysegul Bulut |
| author_sort | Mehmet Burak Ates |
| collection | DOAJ |
| description | <b>Aim: </b>The purpose was to investigate the effects of various dosages of
thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced
hepatotoxicity in rats from a pathological standpoint.<p>
<b>Materials and Methods:</b> Eighty male Wistar Albino rats were used in
this study. Animals were divided in to 10 groups: Control (physiological
saline, PO); Doxorubicin (physiological saline, PO and Dox,15mg/kg Dox in
10th days, IP); Thymoquinone -5 (TQ-5, 5 mg/kg TQ, PO); TQ-20 (20 mg/
kg TQ, PO); Resveratrol-5 (Res-5, 5 mg/kg Res, PO); Res-20 (20 mg/kg Res,
PO); Dox+TQ-5 ; Dox+TQ-20; Dox+Res-5; Dox+Res-20. After the 21-day
experiment, 6 replicates were randomly selected from the groups. After
weighing the body weight, the livers of the euthanized rats were dissected
and weighed. Routine tissue processing processes were applied to liver
samples. Hepatocyte degeneration, necrosis/apoptosis, bile duct hyperplasia,
dissociation, congestion, karyomegaly, mononuclear cell infiltration, binuclear
hepatocytes, and mitosis were all examined microscopically, and a liver total
lesion score was calculated<p>
<b>Results:</b> Dox treatment increased relative liver weight, but the TQ and Res
groups prevented this increase (p<0.05). The liver total lesion score, which
increased with Dox, was shown to be lower in the TQ and Res groups (p<0.05).
However, the Dox+TQ-5, Dox+TQ-20, and Dox+Res-20 groups, showed no
amelioration in necrosis/apoptosis.<p>
<b>Conclusion: </b>TQ and Res (5 and 20 mg/kg) decreased the total liver lesion
score induced by Dox. Although TQ and RES diminish degeneration and
inflammation, their poor protective effects on necrosis/apoptosis, one of the
key criteria, were considered as a limiting cause for their uncontrolled usage. |
| format | Article |
| id | doaj-art-c5138dc7dbed4f0a9a04a6cad026ad39 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2022-09-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-c5138dc7dbed4f0a9a04a6cad026ad392025-01-03T01:36:18ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532022-09-013831501581398Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicinMehmet Burak Ates0Ozgur Ozdemir1Ali Erdem Ozturk2Mustafa Numan Bucak3Aysegul Bulut4Selcuk University, Veterinary Faculty, Department of Pathology, Konya, TurkeySelcuk University, Veterinary Faculty, Department of Pathology, Konya, TurkeyErciyes University, Veterinary Faculty, Department of Reproduction and Artificial Insemination, Kayseri, TurkeySelcuk University, Veterinary Faculty, Department of Reproduction and Artificial Insemination, Konya, TurkeySelcuk University, Veterinary Faculty, Department of Pathology, Konya, Turkey<b>Aim: </b>The purpose was to investigate the effects of various dosages of thymoquinone and resveratrol (5 and 20 mg/kg) on doxorubicin-induced hepatotoxicity in rats from a pathological standpoint.<p> <b>Materials and Methods:</b> Eighty male Wistar Albino rats were used in this study. Animals were divided in to 10 groups: Control (physiological saline, PO); Doxorubicin (physiological saline, PO and Dox,15mg/kg Dox in 10th days, IP); Thymoquinone -5 (TQ-5, 5 mg/kg TQ, PO); TQ-20 (20 mg/ kg TQ, PO); Resveratrol-5 (Res-5, 5 mg/kg Res, PO); Res-20 (20 mg/kg Res, PO); Dox+TQ-5 ; Dox+TQ-20; Dox+Res-5; Dox+Res-20. After the 21-day experiment, 6 replicates were randomly selected from the groups. After weighing the body weight, the livers of the euthanized rats were dissected and weighed. Routine tissue processing processes were applied to liver samples. Hepatocyte degeneration, necrosis/apoptosis, bile duct hyperplasia, dissociation, congestion, karyomegaly, mononuclear cell infiltration, binuclear hepatocytes, and mitosis were all examined microscopically, and a liver total lesion score was calculated<p> <b>Results:</b> Dox treatment increased relative liver weight, but the TQ and Res groups prevented this increase (p<0.05). The liver total lesion score, which increased with Dox, was shown to be lower in the TQ and Res groups (p<0.05). However, the Dox+TQ-5, Dox+TQ-20, and Dox+Res-20 groups, showed no amelioration in necrosis/apoptosis.<p> <b>Conclusion: </b>TQ and Res (5 and 20 mg/kg) decreased the total liver lesion score induced by Dox. Although TQ and RES diminish degeneration and inflammation, their poor protective effects on necrosis/apoptosis, one of the key criteria, were considered as a limiting cause for their uncontrolled usage.http://eurasianjvetsci.org/pdf.php3?id=1398liverhistopathologydoxorubicinthymoquinoneresveratrol |
| spellingShingle | Mehmet Burak Ates Ozgur Ozdemir Ali Erdem Ozturk Mustafa Numan Bucak Aysegul Bulut Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin Eurasian Journal of Veterinary Sciences liver histopathology doxorubicin thymoquinone resveratrol |
| title | Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| title_full | Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| title_fullStr | Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| title_full_unstemmed | Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| title_short | Histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| title_sort | histopathological evaluation of the effects of thymoquinone and resveratrol on the liver in rats administered doxorubicin |
| topic | liver histopathology doxorubicin thymoquinone resveratrol |
| url | http://eurasianjvetsci.org/pdf.php3?id=1398 |
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