Formation of adaptive immunity against salmonellosis in cows using effector memory cells
The study aimed to investigate how the number of effector memory cells changes under the influence of a Salmonella vaccine antigen in cows. A homogeneous group of 100 Holstein-Friesian cows, kept under the same conditions, had blood samples taken. The blood was collected at four time points: before...
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| Format: | Article |
| Language: | English |
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Zhytomyr National Agroecological University
2024-09-01
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| Series: | Наукові горизонти |
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| Online Access: | https://sciencehorizon.com.ua/en/journals/tom-27-10-2024/formuvannya-adaptivnogo-imunitetu-proti-salmonelozu-u-koriv-z-vikoristannyam-efektornikh-klitin-pam-yati |
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| author | Birzhan Biyashev Saparkhan Zhanabayev Zhumagul Kirkimbaeva Arman Zhylkaydar Gulnur Kuzembekova |
| author_facet | Birzhan Biyashev Saparkhan Zhanabayev Zhumagul Kirkimbaeva Arman Zhylkaydar Gulnur Kuzembekova |
| author_sort | Birzhan Biyashev |
| collection | DOAJ |
| description | The study aimed to investigate how the number of effector memory cells changes under the influence of a Salmonella vaccine antigen in cows. A homogeneous group of 100 Holstein-Friesian cows, kept under the same conditions, had blood samples taken. The blood was collected at four time points: before the first vaccination, and 7, 45, and 56 days after. The cows also received a booster vaccination on days 8-10. They were immunised with a polyvalent vaccine against livestock salmonellosis in Kazakhstan. Blood was separated into plasma and cellular fractions by centrifugation at 1,500 rpm for 10 minutes. The cellular fraction was then analysed by flow cytometry to determine the number of CD4+, CD8+, and γδ T-cell subpopulations at the four time points: before vaccination, and at 7-, 45-, and 56-days post-vaccination. Analysis of over 10,000 cells from each sample was conducted using FlowJo software. The data showed that the population of CD4+ and CD8+ T-lymphocytes and γδ T-cells increased 1.5 times by day 7 after the initial vaccination. The cows then received a booster dose on days 8-10, and by day 45 after the first vaccination, the CD4+ and CD8+ T-lymphocyte and γδ T-cell populations had increased threefold. CD45RA+ T-lymphocytes and γδ T-cells demonstrated a steady increase by day 45, followed by a decline in the numbers of T-cells across all phylogenetic groups. Thus, it can be concluded that the primary vaccination stimulates the development of long-term immune memory, while the booster dose triples the number of CD4+, CD8+, and γδ T-cell subpopulations. The findings provide insights into the mechanism of adaptive immunity formation in cows against salmonellosis through the use of effector memory cells and may be applied in developing vaccination strategies for cattle |
| format | Article |
| id | doaj-art-c4898b59b78c4abdacf3587b23fccffb |
| institution | Kabale University |
| issn | 2663-2144 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Zhytomyr National Agroecological University |
| record_format | Article |
| series | Наукові горизонти |
| spelling | doaj-art-c4898b59b78c4abdacf3587b23fccffb2024-11-28T13:29:49ZengZhytomyr National Agroecological UniversityНаукові горизонти2663-21442024-09-01271091910.48077/scihor10.2024.09Formation of adaptive immunity against salmonellosis in cows using effector memory cellsBirzhan Biyashev0https://orcid.org/0000-0003-3603-490XSaparkhan Zhanabayev1https://orcid.org/0000-0002-7025-3113Zhumagul Kirkimbaeva2https://orcid.org/0000-0001-8820-9260Arman Zhylkaydar3https://orcid.org/0000-0003-2439-9792Gulnur Kuzembekova4https://orcid.org/0000-0002-7914-7835Kazakh National Agrarian Research UniversityKazakh National Agrarian Research UniversityKazakh National Agrarian Research UniversityKazakh National Agrarian Research UniversityKazakh National Agrarian Research UniversityThe study aimed to investigate how the number of effector memory cells changes under the influence of a Salmonella vaccine antigen in cows. A homogeneous group of 100 Holstein-Friesian cows, kept under the same conditions, had blood samples taken. The blood was collected at four time points: before the first vaccination, and 7, 45, and 56 days after. The cows also received a booster vaccination on days 8-10. They were immunised with a polyvalent vaccine against livestock salmonellosis in Kazakhstan. Blood was separated into plasma and cellular fractions by centrifugation at 1,500 rpm for 10 minutes. The cellular fraction was then analysed by flow cytometry to determine the number of CD4+, CD8+, and γδ T-cell subpopulations at the four time points: before vaccination, and at 7-, 45-, and 56-days post-vaccination. Analysis of over 10,000 cells from each sample was conducted using FlowJo software. The data showed that the population of CD4+ and CD8+ T-lymphocytes and γδ T-cells increased 1.5 times by day 7 after the initial vaccination. The cows then received a booster dose on days 8-10, and by day 45 after the first vaccination, the CD4+ and CD8+ T-lymphocyte and γδ T-cell populations had increased threefold. CD45RA+ T-lymphocytes and γδ T-cells demonstrated a steady increase by day 45, followed by a decline in the numbers of T-cells across all phylogenetic groups. Thus, it can be concluded that the primary vaccination stimulates the development of long-term immune memory, while the booster dose triples the number of CD4+, CD8+, and γδ T-cell subpopulations. The findings provide insights into the mechanism of adaptive immunity formation in cows against salmonellosis through the use of effector memory cells and may be applied in developing vaccination strategies for cattlehttps://sciencehorizon.com.ua/en/journals/tom-27-10-2024/formuvannya-adaptivnogo-imunitetu-proti-salmonelozu-u-koriv-z-vikoristannyam-efektornikh-klitin-pam-yaticd4+ and cd8+ t-lymphocytescd45ra+ t-lymphocytesimmunological memoryvaccinationcellular immunityhumoral immunity |
| spellingShingle | Birzhan Biyashev Saparkhan Zhanabayev Zhumagul Kirkimbaeva Arman Zhylkaydar Gulnur Kuzembekova Formation of adaptive immunity against salmonellosis in cows using effector memory cells Наукові горизонти cd4+ and cd8+ t-lymphocytes cd45ra+ t-lymphocytes immunological memory vaccination cellular immunity humoral immunity |
| title | Formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| title_full | Formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| title_fullStr | Formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| title_full_unstemmed | Formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| title_short | Formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| title_sort | formation of adaptive immunity against salmonellosis in cows using effector memory cells |
| topic | cd4+ and cd8+ t-lymphocytes cd45ra+ t-lymphocytes immunological memory vaccination cellular immunity humoral immunity |
| url | https://sciencehorizon.com.ua/en/journals/tom-27-10-2024/formuvannya-adaptivnogo-imunitetu-proti-salmonelozu-u-koriv-z-vikoristannyam-efektornikh-klitin-pam-yati |
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