<i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars
<i>Pseudomonas syringae</i> pv. <i>syringae</i> is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses...
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MDPI AG
2024-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/1/87 |
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| author | Claudia Carreras Alan Zamorano Luis Villalobos-González Paula Pimentel Lorena Pizarro María Francisca Beltrán Weier Cui Manuel Pinto Franco Figueroa Carlos Rubilar-Hernández Analia Llanes Assunta Bertaccini Nicola Fiore |
| author_facet | Claudia Carreras Alan Zamorano Luis Villalobos-González Paula Pimentel Lorena Pizarro María Francisca Beltrán Weier Cui Manuel Pinto Franco Figueroa Carlos Rubilar-Hernández Analia Llanes Assunta Bertaccini Nicola Fiore |
| author_sort | Claudia Carreras |
| collection | DOAJ |
| description | <i>Pseudomonas syringae</i> pv. <i>syringae</i> is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to <i>P. syringae</i> pv. <i>syringae</i>, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. <i>P. syringae</i> pv. <i>syringae</i> infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to <i>P. syringae</i> pv. <i>syringae</i> is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to <i>P. syringae</i> pv. <i>syringae</i>. |
| format | Article |
| id | doaj-art-c3e71c85c85f48caa585849d528225f2 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-c3e71c85c85f48caa585849d528225f22025-01-10T13:19:43ZengMDPI AGPlants2223-77472024-12-011418710.3390/plants14010087<i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry CultivarsClaudia Carreras0Alan Zamorano1Luis Villalobos-González2Paula Pimentel3Lorena Pizarro4María Francisca Beltrán5Weier Cui6Manuel Pinto7Franco Figueroa8Carlos Rubilar-Hernández9Analia Llanes10Assunta Bertaccini11Nicola Fiore12Facultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, La Pintana 8820808, ChileFacultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, La Pintana 8820808, ChileCentro de Estudios Avanzados en Fruticultura (CEAF), Rengo 2940000, ChileCentro de Estudios Avanzados en Fruticultura (CEAF), Rengo 2940000, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando 3070000, ChilePinto Piga Seeds S.A., Joaquín Rodríguez 7317, Macul 7820206, ChileFacultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, La Pintana 8820808, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando 3070000, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando 3070000, ChileInstituto de Ciencias Agroalimentarias, Animales y Ambientales, Universidad de O’Higgins, San Fernando 3070000, ChileLaboratorio de Fisiología Vegetal-Interacción Planta-Ambiente, Departamento de Ciencias Naturales, Universidad Nacional de Río Cuarto, Ruta Nac. 36—Km. 601, Río Cuarto X5804BYA, Córdoba, ArgentinaDepartment of Agricultural and Food Sciences, <i>Alma Mater Studiorum</i>—University of Bologna, 40127 Bologna, ItalyFacultad de Ciencias Agronómicas, Departamento de Sanidad Vegetal, Universidad de Chile, La Pintana 8820808, Chile<i>Pseudomonas syringae</i> pv. <i>syringae</i> is the main causal agent of bacterial canker in sweet cherry in Chile, causing significant economic losses. Cultivars exhibit diverse susceptibility in the field and the molecular mechanisms underlying the differential responses remain unclear. RNA-seq analysis was performed to characterize the transcriptomic response in cultivars Santina and Bing (less and more susceptible to <i>P. syringae</i> pv. <i>syringae</i>, respectively) after 1 and 7 days post-inoculation (dpi) with the bacterium. Symptoms of bacterial canker became evident from the fifth day. At 1 dpi, cultivar Santina showed a faster response to infection and a larger number of differentially expressed genes (DEGs) than cultivar Bing. At 7 dpi, cultivar Bing almost doubled its DEGs, while cultivar Santina tended to the normal DEG levels. <i>P. syringae</i> pv. <i>syringae</i> infection downregulated the expressions of key genes of the photosynthesis process at 1 dpi in the less susceptible cultivar. The results suggest that the difference in susceptibility to <i>P. syringae</i> pv. <i>syringae</i> is linked to the timeliness of pathogen recognition, limiting the bacteria’s dispersion through modeling its cell wall, and regulation of genes encoding photosynthesis pathway. Through this study, it has been possible to progress the knowledge of relevant factors related to the susceptibility of the two studied cherry cultivars to <i>P. syringae</i> pv. <i>syringae</i>.https://www.mdpi.com/2223-7747/14/1/87plant pathogenic bacteriatranscriptomeplant–pathogen interaction |
| spellingShingle | Claudia Carreras Alan Zamorano Luis Villalobos-González Paula Pimentel Lorena Pizarro María Francisca Beltrán Weier Cui Manuel Pinto Franco Figueroa Carlos Rubilar-Hernández Analia Llanes Assunta Bertaccini Nicola Fiore <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars Plants plant pathogenic bacteria transcriptome plant–pathogen interaction |
| title | <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars |
| title_full | <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars |
| title_fullStr | <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars |
| title_full_unstemmed | <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars |
| title_short | <i>Pseudomonas syringae</i> Pathovar <i>syringae</i> Infection Reveals Different Defense Mechanisms in Two Sweet Cherry Cultivars |
| title_sort | i pseudomonas syringae i pathovar i syringae i infection reveals different defense mechanisms in two sweet cherry cultivars |
| topic | plant pathogenic bacteria transcriptome plant–pathogen interaction |
| url | https://www.mdpi.com/2223-7747/14/1/87 |
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