Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid

Objective The active metabolite of vitamin A, all-trans retinoic acid (ATRA), is involved in the proliferation and differentiation of granulosa cells, and promotes the follicular development, oocyte maturation, and ovulation in mammals. This study aims to investigate the ATRA induced potential long...

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Main Authors: Jinzhu Meng, Xiuwen Chen, Huabiao Wang, Yixuan Mi, Runsheng Zhou, Hongliang Zhang
Format: Article
Language:English
Published: Asian-Australasian Association of Animal Production Societies 2025-02-01
Series:Animal Bioscience
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Online Access:http://www.animbiosci.org/upload/pdf/ab-24-0363.pdf
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author Jinzhu Meng
Xiuwen Chen
Huabiao Wang
Yixuan Mi
Runsheng Zhou
Hongliang Zhang
author_facet Jinzhu Meng
Xiuwen Chen
Huabiao Wang
Yixuan Mi
Runsheng Zhou
Hongliang Zhang
author_sort Jinzhu Meng
collection DOAJ
description Objective The active metabolite of vitamin A, all-trans retinoic acid (ATRA), is involved in the proliferation and differentiation of granulosa cells, and promotes the follicular development, oocyte maturation, and ovulation in mammals. This study aims to investigate the ATRA induced potential long noncoding RNAs (lncRNAs) that regulate the expression of genes associated with granulosa cell proliferation and follicular development. Methods The lncRNA and mRNA profiles of porcine granulosa cells from ATRA treatment and control group in vitro were constructed through RNA sequencing. Meanwhile, the sequencing data were verified using quantitative polymerase chain reaction (qPCR). Results A total of 86 differentially expressed lncRNAs and 128 differentially expressed genes (DEGs) were detected in granulosa cells after ATRA treatment. The quantitative real-time PCR (qRT-PCR) results were consistent with the RNA-seq data. Functional annotation analysis revealed that the DEGs were remarkably enriched in ovary function and reproduction which contained FoxO, Hippo, Oocyte meiosis, mammalian target of rapamycin signaling pathway, as well as several pathways associated with hormone regulation like oxytocin signaling pathway and steroid hormone biosynthesis. Moreover, an interaction network of lncRNAs and their cis-target DEGs was constructed, and 7 differentially expressed lncRNAs and 6 cis-target DEGs were enriched in ovarian steroidogenesis and reproduction. Conclusion These findings expand the lncRNA catalogue and provide a basis for further studies on the mechanism of ATRA-mediated lncRNA regulation of follicular development in pigs.
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spelling doaj-art-c3c3915e315f46ae8f7c98e0f25771c02025-01-03T04:14:22ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-02-0138226727710.5713/ab.24.036325332Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acidJinzhu Meng0Xiuwen Chen1Huabiao Wang2Yixuan Mi3Runsheng Zhou4Hongliang Zhang5 College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, ChinaObjective The active metabolite of vitamin A, all-trans retinoic acid (ATRA), is involved in the proliferation and differentiation of granulosa cells, and promotes the follicular development, oocyte maturation, and ovulation in mammals. This study aims to investigate the ATRA induced potential long noncoding RNAs (lncRNAs) that regulate the expression of genes associated with granulosa cell proliferation and follicular development. Methods The lncRNA and mRNA profiles of porcine granulosa cells from ATRA treatment and control group in vitro were constructed through RNA sequencing. Meanwhile, the sequencing data were verified using quantitative polymerase chain reaction (qPCR). Results A total of 86 differentially expressed lncRNAs and 128 differentially expressed genes (DEGs) were detected in granulosa cells after ATRA treatment. The quantitative real-time PCR (qRT-PCR) results were consistent with the RNA-seq data. Functional annotation analysis revealed that the DEGs were remarkably enriched in ovary function and reproduction which contained FoxO, Hippo, Oocyte meiosis, mammalian target of rapamycin signaling pathway, as well as several pathways associated with hormone regulation like oxytocin signaling pathway and steroid hormone biosynthesis. Moreover, an interaction network of lncRNAs and their cis-target DEGs was constructed, and 7 differentially expressed lncRNAs and 6 cis-target DEGs were enriched in ovarian steroidogenesis and reproduction. Conclusion These findings expand the lncRNA catalogue and provide a basis for further studies on the mechanism of ATRA-mediated lncRNA regulation of follicular development in pigs.http://www.animbiosci.org/upload/pdf/ab-24-0363.pdfall-trans retinoic acid (atra)follicular developmentgranulosa cellspig
spellingShingle Jinzhu Meng
Xiuwen Chen
Huabiao Wang
Yixuan Mi
Runsheng Zhou
Hongliang Zhang
Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
Animal Bioscience
all-trans retinoic acid (atra)
follicular development
granulosa cells
pig
title Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
title_full Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
title_fullStr Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
title_full_unstemmed Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
title_short Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid
title_sort porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncrnas and mrnas in response to all trans retinoic acid
topic all-trans retinoic acid (atra)
follicular development
granulosa cells
pig
url http://www.animbiosci.org/upload/pdf/ab-24-0363.pdf
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