A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

A HABA dye-based colorimetric assay to detect unoccupied biotin binding sites in an avidin-containing fusion protein

Avidin-biotin binding, the most robust non-covalent protein-ligand interaction occurring in nature, has wide-ranging applications in biotechnology. A frequent challenge in these applications is accurately determining the number of unoccupied biotin binding sites in avidin-containing fusion proteins....

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Bibliographic Details
Main Authors: Sonia Mukherjee, Pierre Leblanc, Mark C Poznansky, Ann E Sluder
Format: Article
Language:English
Published: Taylor & Francis Group 2024-10-01
Series:BioTechniques
Subjects:
avidin
binding assay
binding site occupancy
biotin
fusion protein
self-assembling vaccine
Online Access:https://www.tandfonline.com/doi/10.1080/07366205.2024.2397288
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https://www.tandfonline.com/doi/10.1080/07366205.2024.2397288

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