circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression
Abstract Background Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs). Methods The circular structure of circVEGFA was validated by...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-025-01738-8 |
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| author | Xinxin Qin Jinbi Zhang Chao Yin Fan Li Wenjie Li Xiaolong Cheng Xing Du Qifa Li Zengxiang Pan |
| author_facet | Xinxin Qin Jinbi Zhang Chao Yin Fan Li Wenjie Li Xiaolong Cheng Xing Du Qifa Li Zengxiang Pan |
| author_sort | Xinxin Qin |
| collection | DOAJ |
| description | Abstract Background Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs). Methods The circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA. Results: In this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs). Conclusions The study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia. |
| format | Article |
| id | doaj-art-c38a6d0b08b9475db4a198d39d0b6c22 |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-c38a6d0b08b9475db4a198d39d0b6c222025-08-20T03:46:16ZengBMCJournal of Ovarian Research1757-22152025-07-0118111310.1186/s13048-025-01738-8circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expressionXinxin Qin0Jinbi Zhang1Chao Yin2Fan Li3Wenjie Li4Xiaolong Cheng5Xing Du6Qifa Li7Zengxiang Pan8College of Animal Science and Technology, Nanjing Agriculture UniversityCollege of Animal Science and Food Engineering, Jinling Institute of TechnologyCollege of Animal Science and Food Engineering, Jinling Institute of TechnologyCollege of Animal Science and Food Engineering, Jinling Institute of TechnologyCollege of Animal Science and Technology, Nanjing Agriculture UniversityCollege of Animal Science and Technology, Nanjing Agriculture UniversityCollege of Animal Science and Technology, Nanjing Agriculture UniversityCollege of Animal Science and Technology, Nanjing Agriculture UniversityCollege of Animal Science and Technology, Nanjing Agriculture UniversityAbstract Background Follicular atresia is a major determinant of ovarian failure in multiparous sows. Non-coding RNAs (ncRNAs) play an important role in the regulatory mechanisms controlling apoptosis within ovarian granulosa cells (GCs). Methods The circular structure of circVEGFA was validated by RNase R and actinomycin D treatments. The function of circVEGFA during apoptosis in GCs was investigated by si-RNA transfection. Furthermore, competitive binding of circVEGFA and TMX4 to miR-21-3p was confirmed by a dual-luciferase reporter gene assay and co-transfection with their inhibitors or siRNA. Results: In this study, we present a novel circular RNA (circRNA), circVEGFA, which shows significantly reduced expression in atretic follicles (AFs) compared to healthy follicles (HFs). Conclusions The study demonstrates that circVEGFA increases TMX4 expression and inhibits apoptosis in GCs through competitive binding to miR-21-3p. This study contributes to the understanding of circRNA regulation after follicular atresia.https://doi.org/10.1186/s13048-025-01738-8Follicular atresiaGranulosa cell apoptosiscircRNAmiRNATMX4 |
| spellingShingle | Xinxin Qin Jinbi Zhang Chao Yin Fan Li Wenjie Li Xiaolong Cheng Xing Du Qifa Li Zengxiang Pan circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression Journal of Ovarian Research Follicular atresia Granulosa cell apoptosis circRNA miRNA TMX4 |
| title | circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression |
| title_full | circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression |
| title_fullStr | circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression |
| title_full_unstemmed | circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression |
| title_short | circVEGFA inhibits apoptosis in porcine ovarian granulosa cells by binding to miR-21-3p and up-regulating TMX4 expression |
| title_sort | circvegfa inhibits apoptosis in porcine ovarian granulosa cells by binding to mir 21 3p and up regulating tmx4 expression |
| topic | Follicular atresia Granulosa cell apoptosis circRNA miRNA TMX4 |
| url | https://doi.org/10.1186/s13048-025-01738-8 |
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