Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes
Abstract Fruit of most apple varieties soften after harvest, and although the hormone ethylene is known to induce softening, the associated pathway is not well resolved. In this study, it is determined that MdEAEL1 (Ethylene‐activated E3 ubiquitin Like 1) is specifically expressed during apple fruit...
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Wiley
2025-06-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202417393 |
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| author | Tong Li Li Liu Guangxin Yang Yingcong Cai Yingda Wang Bowen Sun Le Sun Weiting Liu Aide Wang |
| author_facet | Tong Li Li Liu Guangxin Yang Yingcong Cai Yingda Wang Bowen Sun Le Sun Weiting Liu Aide Wang |
| author_sort | Tong Li |
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| description | Abstract Fruit of most apple varieties soften after harvest, and although the hormone ethylene is known to induce softening, the associated pathway is not well resolved. In this study, it is determined that MdEAEL1 (Ethylene‐activated E3 ubiquitin Like 1) is specifically expressed during apple fruit postharvest storage, activated by ethylene, and interacts with the transcription factor MdZFP3 (zinc finger protein3). MdZFP3 is found to rely on an EAR (ethylene‐responsive element binding factor‐associated amphiphilic repression) motif to form a transcriptional repression complex with MdTPL4 (TOPLESS4)‐MdHDA19 (histone deacetylase19), thereby downregulating the histone acetylation levels of the promoters of a range of cell wall degradation‐related genes and inhibiting their transcription. MdEAEL1 ubiquitinates and degrades MdZFP3, leading to the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex. This process promotes the transcription of cell wall degradation‐related genes, resulting in fruit softening during storage. Furthermore, the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex, mediated by MdEAEL1, upregulates the transcription of MdEAEL1 itself, creating a feedback loop that further promotes softening. This study elucidates the interplay between post‐translational modifications of a transcription factor and its epigenetic modification to regulate fruit softening, and highlights the complexity of ethylene‐induced softening. |
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| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-06-01 |
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| spelling | doaj-art-c1d4b6cc13ce4fd6b79a1ae6c843f5942025-08-20T03:44:51ZengWileyAdvanced Science2198-38442025-06-011222n/an/a10.1002/advs.202417393Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor ComplexesTong Li0Li Liu1Guangxin Yang2Yingcong Cai3Yingda Wang4Bowen Sun5Le Sun6Weiting Liu7Aide Wang8Key Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaLiaoning Institute of Pomology Xiongyue 115009 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaKey Laboratory of Fruit Postharvest Biology (Liaoning Province) Key Laboratory of Protected Horticulture (Ministry of Education) National & Local Joint Engineering Research Center of Northern Horticultural Facilities Design & Application Technology (Liaoning) College of Horticulture Shenyang Agricultural University Shenyang 110866 ChinaAbstract Fruit of most apple varieties soften after harvest, and although the hormone ethylene is known to induce softening, the associated pathway is not well resolved. In this study, it is determined that MdEAEL1 (Ethylene‐activated E3 ubiquitin Like 1) is specifically expressed during apple fruit postharvest storage, activated by ethylene, and interacts with the transcription factor MdZFP3 (zinc finger protein3). MdZFP3 is found to rely on an EAR (ethylene‐responsive element binding factor‐associated amphiphilic repression) motif to form a transcriptional repression complex with MdTPL4 (TOPLESS4)‐MdHDA19 (histone deacetylase19), thereby downregulating the histone acetylation levels of the promoters of a range of cell wall degradation‐related genes and inhibiting their transcription. MdEAEL1 ubiquitinates and degrades MdZFP3, leading to the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex. This process promotes the transcription of cell wall degradation‐related genes, resulting in fruit softening during storage. Furthermore, the disassembly of the MdZFP3‐MdTPL4‐MdHDA19 transcriptional repression complex, mediated by MdEAEL1, upregulates the transcription of MdEAEL1 itself, creating a feedback loop that further promotes softening. This study elucidates the interplay between post‐translational modifications of a transcription factor and its epigenetic modification to regulate fruit softening, and highlights the complexity of ethylene‐induced softening.https://doi.org/10.1002/advs.202417393apple fruitethylenefruit softening |
| spellingShingle | Tong Li Li Liu Guangxin Yang Yingcong Cai Yingda Wang Bowen Sun Le Sun Weiting Liu Aide Wang Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes Advanced Science apple fruit ethylene fruit softening |
| title | Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes |
| title_full | Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes |
| title_fullStr | Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes |
| title_full_unstemmed | Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes |
| title_short | Ethylene‐Activated E3 Ubiquitin Ligase MdEAEL1 Promotes Apple Fruit Softening by Facilitating the Dissociation of Transcriptional Repressor Complexes |
| title_sort | ethylene activated e3 ubiquitin ligase mdeael1 promotes apple fruit softening by facilitating the dissociation of transcriptional repressor complexes |
| topic | apple fruit ethylene fruit softening |
| url | https://doi.org/10.1002/advs.202417393 |
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