Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet
A novel green ultra high performance liquid chromatography (UHPLC) method was validated and estimated the measurement uncertainty for simultaneous determination of metoprolol tartrate (MET) and hydrochlorothiazide (HCT) in binary tablet. A Zorbax® SB-C18 column with isocratic elution (flow rate 0.9 ...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Journal of Taibah University for Science |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/16583655.2024.2339010 |
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| author | Claudia Vilela de Oliveira Adriano Peron Anas Rashid Frank Alonso Gavilano Fajardo Felipe Rebello Lourenço Marina Franco Maggi Tavares María Segunda Aurora Prado |
| author_facet | Claudia Vilela de Oliveira Adriano Peron Anas Rashid Frank Alonso Gavilano Fajardo Felipe Rebello Lourenço Marina Franco Maggi Tavares María Segunda Aurora Prado |
| author_sort | Claudia Vilela de Oliveira |
| collection | DOAJ |
| description | A novel green ultra high performance liquid chromatography (UHPLC) method was validated and estimated the measurement uncertainty for simultaneous determination of metoprolol tartrate (MET) and hydrochlorothiazide (HCT) in binary tablet. A Zorbax® SB-C18 column with isocratic elution (flow rate 0.9 mL min−1) at 25°C was used. Analytes and HCT degradation product were separated in approximately 1 min using acetonitrile:water:triethylamine (17:83:0.2, v/v) as mobile phase. Analytical curves were linear with (R2 > 0.99 for MET and HCT) with detection limits of 2.42 and 1.05 µg mL−1 for MET and HCT, respectively. Precision measurements showed RSD% from 0.39 to 1.2% and method accuracy by recovery tests was 100 ± 2%. Measurement uncertainties using Eurachem procedure were 100.1 ± 2.8% and 100.3 ± 2.4% for MET and HCT, respectively. This UHPLC method was accurate, precise, linear and selective, making it suitable for pharmaceutical quality control. It also proved eco-friendly compared to other reported methods. |
| format | Article |
| id | doaj-art-c1abe8a0144243a9b058afb8e7d66059 |
| institution | Kabale University |
| issn | 1658-3655 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj-art-c1abe8a0144243a9b058afb8e7d660592024-12-17T11:38:48ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552024-12-0118110.1080/16583655.2024.2339010Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tabletClaudia Vilela de Oliveira0Adriano Peron1Anas Rashid2Frank Alonso Gavilano Fajardo3Felipe Rebello Lourenço4Marina Franco Maggi Tavares5María Segunda Aurora Prado6Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilAB Sciex Comércio de Instrumentos Laboratoriais Ltda, São Paulo, BrazilDepartment of Neuroscience “Rita Levi Montalcini”, School of Medicine and Surgery, University of Torino, Torino, ItalyDepartment of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilDepartment of Fundamental Chemistry, Institute of Chemistry, University of São Paulo, São Paulo, BrazilDepartment of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, BrazilA novel green ultra high performance liquid chromatography (UHPLC) method was validated and estimated the measurement uncertainty for simultaneous determination of metoprolol tartrate (MET) and hydrochlorothiazide (HCT) in binary tablet. A Zorbax® SB-C18 column with isocratic elution (flow rate 0.9 mL min−1) at 25°C was used. Analytes and HCT degradation product were separated in approximately 1 min using acetonitrile:water:triethylamine (17:83:0.2, v/v) as mobile phase. Analytical curves were linear with (R2 > 0.99 for MET and HCT) with detection limits of 2.42 and 1.05 µg mL−1 for MET and HCT, respectively. Precision measurements showed RSD% from 0.39 to 1.2% and method accuracy by recovery tests was 100 ± 2%. Measurement uncertainties using Eurachem procedure were 100.1 ± 2.8% and 100.3 ± 2.4% for MET and HCT, respectively. This UHPLC method was accurate, precise, linear and selective, making it suitable for pharmaceutical quality control. It also proved eco-friendly compared to other reported methods.https://www.tandfonline.com/doi/10.1080/16583655.2024.2339010UHPLCmetoprolol tartratehydrochlorothiazidemeasurement uncertaintygreenness |
| spellingShingle | Claudia Vilela de Oliveira Adriano Peron Anas Rashid Frank Alonso Gavilano Fajardo Felipe Rebello Lourenço Marina Franco Maggi Tavares María Segunda Aurora Prado Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet Journal of Taibah University for Science UHPLC metoprolol tartrate hydrochlorothiazide measurement uncertainty greenness |
| title | Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| title_full | Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| title_fullStr | Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| title_full_unstemmed | Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| title_short | Validation, measurement uncertainty estimation and evaluation of UHPLC greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| title_sort | validation measurement uncertainty estimation and evaluation of uhplc greenness for simultaneous determination of metoprolol tartrate and hydrochlorothiazide in binary tablet |
| topic | UHPLC metoprolol tartrate hydrochlorothiazide measurement uncertainty greenness |
| url | https://www.tandfonline.com/doi/10.1080/16583655.2024.2339010 |
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