两孔一步法同时检测HBsAg与抗-HBs的ELISA技术
以一步夹心法检测HBsAg的ELISA技术为基础,结合中和试验原理,提出了两孔一步法同时检测HBsAg与抗-HBs的ELISA技术。对已知RIA法为HBsAg阳性血清108份、抗-HBs阳性且S/N>10的血清395份、HBsAg与抗-HBs均阴性的血清167份,用本法检测,结果符合RIA法的分别为106份(符合率98.14%),385份(97.46%)和158份(94.61%),对乙肝疫苗免疫前的各类健康人血清424份及经乙肝疫苗三针免疫后1—2个月的血清359份,用本法及RIA法平行测试HBsAg与抗-HBs,结果两法总的符合率分别为89.39%(379份)87.30%(282份)。...
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Format: | Article |
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Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
1989-01-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
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Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/43594310/ |
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