Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21

Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (P...

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Bibliographic Details
Main Authors:	Intan Taufik, Rizal Fanany, Agika Manjaswari, Fenryco Pratama
Format:	Article
Language:	English
Published:	Bogor Agricultural University 2024-11-01
Series:	Hayati Journal of Biosciences
Online Access:	https://journal.ipb.ac.id/index.php/hayati/article/view/58465
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