Cloning and Optimized Expression of Bst DNA Polymerase from Geobacillus stearothermophillus in Escherichia coli BL21

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (P...

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Bibliographic Details
Main Authors: Intan Taufik, Rizal Fanany, Agika Manjaswari, Fenryco Pratama
Format: Article
Language:English
Published: Bogor Agricultural University 2024-11-01
Series:Hayati Journal of Biosciences
Online Access:https://journal.ipb.ac.id/index.php/hayati/article/view/58465
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