Differential expression of microRNA-3127-5p and its molecular mechanisms in polycystic ovary syndrome
INTRODUCTION: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Via Medica
2025-08-01
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| Series: | Endokrynologia Polska |
| Subjects: | |
| Online Access: | https://journals.viamedica.pl/endokrynologia_polska/article/view/104875 |
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| Summary: | INTRODUCTION: The present study was undertaken to elucidate the expression status and molecular mechanism underlying microRNA-3127-5p (miR-3127-5p) in polycystic ovary syndrome (PCOS). MATERIAL AND METHODS: A total of 50 PCOS and 50 non-PCOS patients were recruited as research subjects. Quantitative real-time polymerase chain reaction was employed to assess the relative abundances of miR-3127-5p in serum, cumulus cells (CCs), and granulosa cells (GCs) from PCOS patients. Additionally, cellular activities and ferroptosis-related biomarkers were evaluated. Bioinformatics analysis was conducted to identify potential targets of miR-3127-5p. To validate the interaction between miR-3127-5p and sorbin and SH3 domain-containing protein 1 (SORBS1), a luciferase reporter assay was conducted. RESULTS: Enforced miR-3127-5p expression was detected in serum, CCs, and GCs from PCOS patients, demonstrating a robust diagnostic capacity for effectively distinguishing between PCOS and non-PCOS patients. Furthermore, the reduction of miR-3127-5p expression was found to enhance cell viability and inhibit cell apoptosis in human granulosa-like tumor cell line (KGN) and the luteinized granulosa cell line (SVOG) cells. Concurrently, its down-regulation led to attenuated levels of iron, malondialdehyde (MDA), and reactive oxygen species (ROS), while simultaneously increasing the expression of glutathione peroxidase 4 (GPX4). Notably, miR-3127-5p expression exhibited an inverse relationship with SORBS1. Rescue experiments revealed that the effects of downregulated miR-3127-5p expression on cellular behaviors and ferroptosis were reserved through the transfection of si-SORBS1. CONCLUSION: The downregulation of miR-3127-5p expression facilitates cell growth and attenuates ferroptosis by targeting SORBS1, thereby playing a pivotal role in the initiation and development of PCOS. |
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| ISSN: | 0423-104X 2299-8306 |