Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico
Rice husk (Oryza sativa L.) is considered an agroindustrial waste that requires alternative treatment to prevent it from becoming an environmental contaminant. Some recent reports have been able to produce Nanocellulose (CNCs) with this biomass, although with low yields, after using a complex of end...
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Iranian Environmental Mutagen Society
2024-05-01
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| Series: | Journal of Water and Environmental Nanotechnology |
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| Online Access: | https://www.jwent.net/article_713099_123ca9b8b249706ff435528a5eb78938.pdf |
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| author | Ricardo Hernández Pérez René Salgado Delgado Alfredo Olarte Paredes Areli Marlen Salgado Delgado Beatriz Flores Abarca Oswaldo E. Silva Astudillo |
| author_facet | Ricardo Hernández Pérez René Salgado Delgado Alfredo Olarte Paredes Areli Marlen Salgado Delgado Beatriz Flores Abarca Oswaldo E. Silva Astudillo |
| author_sort | Ricardo Hernández Pérez |
| collection | DOAJ |
| description | Rice husk (Oryza sativa L.) is considered an agroindustrial waste that requires alternative treatment to prevent it from becoming an environmental contaminant. Some recent reports have been able to produce Nanocellulose (CNCs) with this biomass, although with low yields, after using a complex of endo and exoenzymes. Work aimed to evaluate the previous effect of natural proteolytic enzymes (Bromelain/Papain) to degrade the fibrous structure of rice husk before obtaining Cellulose and for whitening, an alternative with hydrogen peroxide. In combination with subsequent enzymatic hydrolysis the new complex formed by four reagents: D-(+)-Cellobiose, endo-1,4-ꞵ-D glucanase, ꞵ-glucosidase, and Cellobiohydrolase, for the synthesis of Nanocrystals (CNCs). Satisfactory results indicated that it was possible to soften and modify the plant tissue structure, which allowed a final cellulose yield of 56.35%. The hydrolysis with the enzyme complex applied to Cellulose, achieved the obtaining of CNCs between 200-595 nm, after using the four enzymes in complex, with better results when used in a single phase, achieving a final yield of 1.8 -4.2% nanocellulose with process optimization. |
| format | Article |
| id | doaj-art-b984e679ac7b4206aab5cab71f5a2c9d |
| institution | Kabale University |
| issn | 2476-7204 2476-6615 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Iranian Environmental Mutagen Society |
| record_format | Article |
| series | Journal of Water and Environmental Nanotechnology |
| spelling | doaj-art-b984e679ac7b4206aab5cab71f5a2c9d2025-01-12T09:34:26ZengIranian Environmental Mutagen SocietyJournal of Water and Environmental Nanotechnology2476-72042476-66152024-05-019212413610.22090/jwent.2024.02.01713099Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, MexicoRicardo Hernández Pérez0René Salgado Delgado1Alfredo Olarte Paredes2Areli Marlen Salgado Delgado3Beatriz Flores Abarca4Oswaldo E. Silva Astudillo5National Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoNational Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoNational Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoNational Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoNational Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoNational Technological Institute of Mexico/Technological Institute of Zacatepec, Road No. 27, Col. Center, Zacatepec, Morelos 62780, MexicoRice husk (Oryza sativa L.) is considered an agroindustrial waste that requires alternative treatment to prevent it from becoming an environmental contaminant. Some recent reports have been able to produce Nanocellulose (CNCs) with this biomass, although with low yields, after using a complex of endo and exoenzymes. Work aimed to evaluate the previous effect of natural proteolytic enzymes (Bromelain/Papain) to degrade the fibrous structure of rice husk before obtaining Cellulose and for whitening, an alternative with hydrogen peroxide. In combination with subsequent enzymatic hydrolysis the new complex formed by four reagents: D-(+)-Cellobiose, endo-1,4-ꞵ-D glucanase, ꞵ-glucosidase, and Cellobiohydrolase, for the synthesis of Nanocrystals (CNCs). Satisfactory results indicated that it was possible to soften and modify the plant tissue structure, which allowed a final cellulose yield of 56.35%. The hydrolysis with the enzyme complex applied to Cellulose, achieved the obtaining of CNCs between 200-595 nm, after using the four enzymes in complex, with better results when used in a single phase, achieving a final yield of 1.8 -4.2% nanocellulose with process optimization.https://www.jwent.net/article_713099_123ca9b8b249706ff435528a5eb78938.pdforyza sativa lagroindustrial wasteenzymesbromelainpapain |
| spellingShingle | Ricardo Hernández Pérez René Salgado Delgado Alfredo Olarte Paredes Areli Marlen Salgado Delgado Beatriz Flores Abarca Oswaldo E. Silva Astudillo Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico Journal of Water and Environmental Nanotechnology oryza sativa l agroindustrial waste enzymes bromelain papain |
| title | Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico |
| title_full | Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico |
| title_fullStr | Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico |
| title_full_unstemmed | Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico |
| title_short | Combined Hydrolysis to Yield Increase of Cellulose and (CNCs), Starting from Agroindustrial Rice Husk Waste in Morelos, Mexico |
| title_sort | combined hydrolysis to yield increase of cellulose and cncs starting from agroindustrial rice husk waste in morelos mexico |
| topic | oryza sativa l agroindustrial waste enzymes bromelain papain |
| url | https://www.jwent.net/article_713099_123ca9b8b249706ff435528a5eb78938.pdf |
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