<i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase
This paper explored the in vitro inhibitory mechanism of polyphenol-rich rose extracts (REs) from an edible rose flower against α-glucosidase using multispectral and molecular docking techniques. Results showed that REs had an inhibitory effect on α-Glu activity (IC<sub>50</sub> of 1.96...
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2024-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/21/3421 |
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| author | Chao Zhang Ming Feng Bimal Chitrakar Fan Yang Benxi Wei Bo Wang Cunshan Zhou Haile Ma Xianli Gao Baoguo Xu |
| author_facet | Chao Zhang Ming Feng Bimal Chitrakar Fan Yang Benxi Wei Bo Wang Cunshan Zhou Haile Ma Xianli Gao Baoguo Xu |
| author_sort | Chao Zhang |
| collection | DOAJ |
| description | This paper explored the in vitro inhibitory mechanism of polyphenol-rich rose extracts (REs) from an edible rose flower against α-glucosidase using multispectral and molecular docking techniques. Results showed that REs had an inhibitory effect on α-Glu activity (IC<sub>50</sub> of 1.96 μg/mL); specifically, the samples pretreated by tri-frequency ultrasound (20/40/60 kHz) exhibited a significantly (<i>p</i> < 0.05) stronger inhibitory effect on α-Glu activity with an IC<sub>50</sub> of 1.33 μg/mL. The Lineweaver–Burk assay indicated that REs were mixed-type inhibitors and could statically quench the endogenous fluorescence of α-Glu. REs increased the chance of polypeptide chain misfolding by altering the microenvironment around tryptophan and tyrosine residues and disrupting the natural conformation of the enzyme. Molecular docking results showed that polyhydroxy phenolics had a high fit to the active site of α-Glu, so REs with high polymerization and numerous phenolic hydroxyl groups had a stronger inhibitory effect. Therefore, this study provides new insights into polyphenol-rich REs as potential α-glucosidase inhibitors. |
| format | Article |
| id | doaj-art-b9678eccfaed4e48a62f8ca843cd83a5 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-b9678eccfaed4e48a62f8ca843cd83a52024-11-08T14:36:12ZengMDPI AGFoods2304-81582024-10-011321342110.3390/foods13213421<i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-GlucosidaseChao Zhang0Ming Feng1Bimal Chitrakar2Fan Yang3Benxi Wei4Bo Wang5Cunshan Zhou6Haile Ma7Xianli Gao8Baoguo Xu9School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaCollege of Food Science and Technology, Hebei Agricultural University, Baoding 071001, ChinaCollege of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, ChinaThis paper explored the in vitro inhibitory mechanism of polyphenol-rich rose extracts (REs) from an edible rose flower against α-glucosidase using multispectral and molecular docking techniques. Results showed that REs had an inhibitory effect on α-Glu activity (IC<sub>50</sub> of 1.96 μg/mL); specifically, the samples pretreated by tri-frequency ultrasound (20/40/60 kHz) exhibited a significantly (<i>p</i> < 0.05) stronger inhibitory effect on α-Glu activity with an IC<sub>50</sub> of 1.33 μg/mL. The Lineweaver–Burk assay indicated that REs were mixed-type inhibitors and could statically quench the endogenous fluorescence of α-Glu. REs increased the chance of polypeptide chain misfolding by altering the microenvironment around tryptophan and tyrosine residues and disrupting the natural conformation of the enzyme. Molecular docking results showed that polyhydroxy phenolics had a high fit to the active site of α-Glu, so REs with high polymerization and numerous phenolic hydroxyl groups had a stronger inhibitory effect. Therefore, this study provides new insights into polyphenol-rich REs as potential α-glucosidase inhibitors.https://www.mdpi.com/2304-8158/13/21/3421polyphenolsα-glucosidaseinhibitory mechanismspectroscopymolecular docking |
| spellingShingle | Chao Zhang Ming Feng Bimal Chitrakar Fan Yang Benxi Wei Bo Wang Cunshan Zhou Haile Ma Xianli Gao Baoguo Xu <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase Foods polyphenols α-glucosidase inhibitory mechanism spectroscopy molecular docking |
| title | <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase |
| title_full | <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase |
| title_fullStr | <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase |
| title_full_unstemmed | <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase |
| title_short | <i>In Vitro</i> Inhibitory Mechanism of Polyphenol Extracts from Multi-Frequency Power Ultrasound-Pretreated Rose Flower Against α-Glucosidase |
| title_sort | i in vitro i inhibitory mechanism of polyphenol extracts from multi frequency power ultrasound pretreated rose flower against α glucosidase |
| topic | polyphenols α-glucosidase inhibitory mechanism spectroscopy molecular docking |
| url | https://www.mdpi.com/2304-8158/13/21/3421 |
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