Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach
This study explores the impact of granular activated carbon (GAC) and L-arginine supplementation on biogas upgrading and acetic acid production employing Clostridium thailandense. GAC and L-arginine concentrations ranged from 0 to 20 g/L and 0 to 5 g/L, respectively, with H2 acting as the electron d...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Carbon Resources Conversion |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2588913324000255 |
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| author | Srisuda Chaikitkaew Nantharat Wongfaed Chonticha Mamimin Sompong O-Thong Alissara Reungsang |
| author_facet | Srisuda Chaikitkaew Nantharat Wongfaed Chonticha Mamimin Sompong O-Thong Alissara Reungsang |
| author_sort | Srisuda Chaikitkaew |
| collection | DOAJ |
| description | This study explores the impact of granular activated carbon (GAC) and L-arginine supplementation on biogas upgrading and acetic acid production employing Clostridium thailandense. GAC and L-arginine concentrations ranged from 0 to 20 g/L and 0 to 5 g/L, respectively, with H2 acting as the electron donor at an H2 to CO2 ratio of 2:1 (v/v). Experiments were conducted at 30 °C with an agitation speed of 150 rpm. Additionally, gene annotation of the C. thailandense genome using Rapid Annotations using Subsystems Technology (RAST) identified genes involved in CO2 to acetic acid conversion. Results indicate that adding 7.5 g/L GAC boosts CH4 purity in biogas, elevating CO2 and H2 consumption efficiencies to 88.3 % and 98.7 %, respectively. This enhancement leads to a CH4 content increase to 93.3 %, accompanied by 0.90 g/L acetic acid production. Conversely, L-arginine demonstrates no significant impact on CO2 conversion. Leveraging RAST, the study identifies hydrogenase genes and NADH-dependent ferredoxin-NADP+ oxidoreductase (Nfn), as crucial for heightened H2 consumption efficiencies and cell growth facilitated by GAC, thus enhancing biogas upgrading efficiency in C. thailandense. This research provides vital insights into optimizing sustainable biogas production through strategic GAC utilization and elucidates the roles of hydrogenase genes and Nfn. |
| format | Article |
| id | doaj-art-b66b51a5fcda4627bd0b1456fdb5b404 |
| institution | Kabale University |
| issn | 2588-9133 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Carbon Resources Conversion |
| spelling | doaj-art-b66b51a5fcda4627bd0b1456fdb5b4042024-12-05T05:21:15ZengKeAi Communications Co., Ltd.Carbon Resources Conversion2588-91332024-12-0174100236Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approachSrisuda Chaikitkaew0Nantharat Wongfaed1Chonticha Mamimin2Sompong O-Thong3Alissara Reungsang4Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, ThailandBiofuel and Biocatalysis Innovation Research Unit, Nakhonsawan Campus, Mahidol University, Nakhonsawan 60130, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand; Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand; Academy of Science, Royal Society of Thailand, Bangkok 10300, Thailand; Corresponding author at: Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.This study explores the impact of granular activated carbon (GAC) and L-arginine supplementation on biogas upgrading and acetic acid production employing Clostridium thailandense. GAC and L-arginine concentrations ranged from 0 to 20 g/L and 0 to 5 g/L, respectively, with H2 acting as the electron donor at an H2 to CO2 ratio of 2:1 (v/v). Experiments were conducted at 30 °C with an agitation speed of 150 rpm. Additionally, gene annotation of the C. thailandense genome using Rapid Annotations using Subsystems Technology (RAST) identified genes involved in CO2 to acetic acid conversion. Results indicate that adding 7.5 g/L GAC boosts CH4 purity in biogas, elevating CO2 and H2 consumption efficiencies to 88.3 % and 98.7 %, respectively. This enhancement leads to a CH4 content increase to 93.3 %, accompanied by 0.90 g/L acetic acid production. Conversely, L-arginine demonstrates no significant impact on CO2 conversion. Leveraging RAST, the study identifies hydrogenase genes and NADH-dependent ferredoxin-NADP+ oxidoreductase (Nfn), as crucial for heightened H2 consumption efficiencies and cell growth facilitated by GAC, thus enhancing biogas upgrading efficiency in C. thailandense. This research provides vital insights into optimizing sustainable biogas production through strategic GAC utilization and elucidates the roles of hydrogenase genes and Nfn.http://www.sciencedirect.com/science/article/pii/S2588913324000255Biogas transformationCarbon capture and utilizationCO2 consumption efficiencyCO2 utilizationSustainable biogas applications |
| spellingShingle | Srisuda Chaikitkaew Nantharat Wongfaed Chonticha Mamimin Sompong O-Thong Alissara Reungsang Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach Carbon Resources Conversion Biogas transformation Carbon capture and utilization CO2 consumption efficiency CO2 utilization Sustainable biogas applications |
| title | Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach |
| title_full | Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach |
| title_fullStr | Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach |
| title_full_unstemmed | Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach |
| title_short | Biogas upgrading towards acetic acid production using Clostridium thailandense supplemented with granular activated carbon (GAC) and L-arginine: A genomic analysis approach |
| title_sort | biogas upgrading towards acetic acid production using clostridium thailandense supplemented with granular activated carbon gac and l arginine a genomic analysis approach |
| topic | Biogas transformation Carbon capture and utilization CO2 consumption efficiency CO2 utilization Sustainable biogas applications |
| url | http://www.sciencedirect.com/science/article/pii/S2588913324000255 |
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