Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing
<i>Bemisia tabaci</i> (<i>Aleyrodidae</i> family) is one of the most damaging pests of numerous crops worldwide. Insecticides, namely pyrethroids and organophosphates, have long been the primary control tools against this pest, resulting in several resistance cases. In Tunisi...
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2025-07-01
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| author | Afef Najjari Chahnez Naccache Nour Abdelkefi Salma Djebbi Amira Souii Brahim Chermiti Mourad Elloumi Maha Mezghani Khemakhem |
| author_facet | Afef Najjari Chahnez Naccache Nour Abdelkefi Salma Djebbi Amira Souii Brahim Chermiti Mourad Elloumi Maha Mezghani Khemakhem |
| author_sort | Afef Najjari |
| collection | DOAJ |
| description | <i>Bemisia tabaci</i> (<i>Aleyrodidae</i> family) is one of the most damaging pests of numerous crops worldwide. Insecticides, namely pyrethroids and organophosphates, have long been the primary control tools against this pest, resulting in several resistance cases. In Tunisia, the two most damaging biotypes of <i>B</i>. <i>tabaci</i>, MEAM1-B and MED-Q, are sympatric, and more concerns about developing resistance keep rising due to the extensive use of insecticides. Here, we aimed to elucidate the molecular mechanism of resistance to pyrethroids and organophosphorus insecticides in two Tunisian populations of <i>B</i>. <i>tabaci</i>, collected respectively on <i>Capsicum annuum</i> and <i>Lantana camara</i>, and then determine the bacterial community associated with insecticide resistance and susceptible biotypes based on 16S rRNA Illumina sequencing. The results showed that the population collected on <i>Capsicum annuum</i> belonged to the MEAM1-B biotype with an insecticide resistance profile. In contrast, the population collected on the <i>Lantana camara</i> belonged to the MED-Q biotype with a sensitive profile. The bacterial communities of the two biotypes were predominantly structured by the Proteobacteria phylum and three genera, including <i>Candidatus Portiera</i>, the secondary facultative symbiont, and <i>Hamiltonella</i>, which were unevenly distributed between the two biotopes. Our results provide the first evidence for insecticide resistance alleles in Tunisian MEAM1-B populations and suggest an association between bacterial community composition within susceptible biotypes and insecticide resistance. |
| format | Article |
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| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-b3c16e6b6ec043b388d0c1f5588c331f2025-08-20T03:58:30ZengMDPI AGMicrobiology Research2036-74812025-07-0116716310.3390/microbiolres16070163Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina SequencingAfef Najjari0Chahnez Naccache1Nour Abdelkefi2Salma Djebbi3Amira Souii4Brahim Chermiti5Mourad Elloumi6Maha Mezghani Khemakhem7Laboratoire de Microbiologie et Biomolécules Actives, Faculty of Sciences of Tunis, LR03ES03, University of Tunis El Manar, Tunis 2092, TunisiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, TunisiaHigh Agronomic Institute of Chott-Mariem, Department of Biological Sciences and Plant Protection, University of Sousse, Sousse 4042, TunisiaCollege of Computing and Information Technology, University of Bisha, Bisha 67714, Saudi ArabiaLaboratory of Biochemistry and Biotechnology (LR01ES05), Faculty of Sciences of Tunis, University of Tunis El Manar, Tunis 1068, Tunisia<i>Bemisia tabaci</i> (<i>Aleyrodidae</i> family) is one of the most damaging pests of numerous crops worldwide. Insecticides, namely pyrethroids and organophosphates, have long been the primary control tools against this pest, resulting in several resistance cases. In Tunisia, the two most damaging biotypes of <i>B</i>. <i>tabaci</i>, MEAM1-B and MED-Q, are sympatric, and more concerns about developing resistance keep rising due to the extensive use of insecticides. Here, we aimed to elucidate the molecular mechanism of resistance to pyrethroids and organophosphorus insecticides in two Tunisian populations of <i>B</i>. <i>tabaci</i>, collected respectively on <i>Capsicum annuum</i> and <i>Lantana camara</i>, and then determine the bacterial community associated with insecticide resistance and susceptible biotypes based on 16S rRNA Illumina sequencing. The results showed that the population collected on <i>Capsicum annuum</i> belonged to the MEAM1-B biotype with an insecticide resistance profile. In contrast, the population collected on the <i>Lantana camara</i> belonged to the MED-Q biotype with a sensitive profile. The bacterial communities of the two biotypes were predominantly structured by the Proteobacteria phylum and three genera, including <i>Candidatus Portiera</i>, the secondary facultative symbiont, and <i>Hamiltonella</i>, which were unevenly distributed between the two biotopes. Our results provide the first evidence for insecticide resistance alleles in Tunisian MEAM1-B populations and suggest an association between bacterial community composition within susceptible biotypes and insecticide resistance.https://www.mdpi.com/2036-7481/16/7/163<i>Bemisia tabaci</i>microbiotanext generation sequencinginsecticides |
| spellingShingle | Afef Najjari Chahnez Naccache Nour Abdelkefi Salma Djebbi Amira Souii Brahim Chermiti Mourad Elloumi Maha Mezghani Khemakhem Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing Microbiology Research <i>Bemisia tabaci</i> microbiota next generation sequencing insecticides |
| title | Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing |
| title_full | Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing |
| title_fullStr | Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing |
| title_full_unstemmed | Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing |
| title_short | Microbiota of the Whitefly <i>Bemisia tabaci</i> (<i>Hemiptera: Aleyrodidae</i>) by 16S rDNA Illumina Sequencing |
| title_sort | microbiota of the whitefly i bemisia tabaci i i hemiptera aleyrodidae i by 16s rdna illumina sequencing |
| topic | <i>Bemisia tabaci</i> microbiota next generation sequencing insecticides |
| url | https://www.mdpi.com/2036-7481/16/7/163 |
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