Development and validation of a sandwich ELISA for equine IL-1β

Abstract In this study, we developed a highly sensitive enzyme-linked immunosorbent assay (ELISA) using newly produced monoclonal antibodies (mAbs) for detecting horse/donkey IL-1β in cell culture medium and serum samples. The mAbs were generated via the use of a KLH-conjugated peptide and purified...

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Bibliographic Details
Main Authors: Saiwen Ma, Xing Guo, Diqiu Liu, Kui Guo, Yuezhi Lin, Xiaojun Wang
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Animal Diseases
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Online Access:https://doi.org/10.1186/s44149-025-00175-w
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