Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than Borrelia spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies...

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Main Authors: Philippe Pérot, Laura Tondeur, Sara Moutailler, Delphine Chrétien, Nicole Corre-Catelin, Muriel Vayssier-Taussat, Marc Eloit, Catherine Chirouze, Céline Cazorla, Laurence Arowas, Elisabeth Botelho-Nevers, Pascale Frey-Klett, Arnaud Fontanet, Clémence Galon, Karine Lacombe, Véronique Perronne, Valentine Piquard, Marie Préau, Costanza Puppo, Marie-Noelle Ungeheuer, Ayla Zayoud
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:One Health
Subjects:
Ticks
Lyme disease
Erythema migrans
Metagenomics
RT-PCR
Pathogen discovery
Online Access:http://www.sciencedirect.com/science/article/pii/S235277142400212X
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