Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study
Dan Li,1– 3,* Qingling Li,4,* Zhen Huang,2 Wenhao Wu,2 Xinyuan Fan,2 Jing Liu,2 Ruoran Li,2 Qi Zhang,2 Xin Su1 1Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, 210000, People’s Republ...
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Dove Medical Press
2025-01-01
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| Series: | Infection and Drug Resistance |
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| author | Li D Li Q Huang Z Wu W Fan X Liu J Li R Zhang Q Su X |
| author_facet | Li D Li Q Huang Z Wu W Fan X Liu J Li R Zhang Q Su X |
| author_sort | Li D |
| collection | DOAJ |
| description | Dan Li,1– 3,* Qingling Li,4,* Zhen Huang,2 Wenhao Wu,2 Xinyuan Fan,2 Jing Liu,2 Ruoran Li,2 Qi Zhang,2 Xin Su1 1Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, 210000, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221000, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, 210002, People’s Republic of China; 4Department of Respiratory and Critical Care Medicine, Xuzhou First People’s Hospital, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin Su, Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, 210000, People’s Republic of China, Email suxinjs@163.com Qi Zhang, Department of Respiratory and Critical Care Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221000, People’s Republic of China, Email zhangqi1107@126.comBackground: tNGS and mNGS are valuable tools for diagnosing pathogens in lower respiratory tract infections (LRTIs), which subsequently influence treatment strategies. However, the impact of tNGS and mNGS on antimicrobial stewardship in patients with LRTIs remains unclear.Methods: Patients diagnosed with LRTIs who underwent tNGS or mNGS between June 2021 and January 2024 were included. Patients who underwent both tNGS and conventional microbiologic tests (CMTs) were grouped into the tNGS group, the others were divided into the mNGS group. Then, the diagnostic efficacy of tNGS and mNGS was compared, along with their impact on antimicrobial management and clinical outcomes.Results: 548 patients with an initial diagnosis of LRTIs who underwent tNGS or mNGS were evaluated. Finally, 321 patients were analyzed, with 117 patients in tNGS group and 204 patients in mNGS group. The overall pathogen detection rates for tNGS and mNGS were 89.74% and 89.71% (P=0.991). The distribution of detected pathogens was similar between tNGS and mNGS, with bacteria being the predominant microorganisms. The proportions of patients who underwent antimicrobial agent changes and received targeted therapy were not significantly different between tNGS and mNGS groups (P=0.270; P=0.893). Additionally, no significant differences were noted in the rates of antibiotic de-escalation, escalation, or changes in the opposite direction (all P> 0.05). The same results was observed in the proportions of patients with addition or reductions in antiviral, antifungal, and antibacterial agents (all P> 0.05). Hospital stays, improvement rate and mortality rate were also similar (all P> 0.05).Conclusion: tNGS and mNGS demonstrate comparable overall pathogen yield rates in patients with LRTIs. Furthermore, tNGS is also comparable to mNGS in terms of adjusting antimicrobial treatments and clinical outcomes, tNGS meets the clinical needs of most patients with LRTIs and can be firstly used for these patients.Keywords: tNGS, mNGS, antimicrobial management, lower respiratory tract infection |
| format | Article |
| id | doaj-art-b0a3b7a3d17f4d2ab1c849e1df490f1e |
| institution | Kabale University |
| issn | 1178-6973 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj-art-b0a3b7a3d17f4d2ab1c849e1df490f1e2025-01-07T16:42:40ZengDove Medical PressInfection and Drug Resistance1178-69732025-01-01Volume 189310598983Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort StudyLi DLi QHuang ZWu WFan XLiu JLi RZhang QSu XDan Li,1– 3,* Qingling Li,4,* Zhen Huang,2 Wenhao Wu,2 Xinyuan Fan,2 Jing Liu,2 Ruoran Li,2 Qi Zhang,2 Xin Su1 1Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, 210000, People’s Republic of China; 2Department of Respiratory and Critical Care Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221000, People’s Republic of China; 3Department of Respiratory and Critical Care Medicine, Jinling Hospital, Nanjing Medical University, Nanjing, 210002, People’s Republic of China; 4Department of Respiratory and Critical Care Medicine, Xuzhou First People’s Hospital, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin Su, Department of Respiratory and Critical Care Medicine, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University, Nanjing, Jiangsu, 210000, People’s Republic of China, Email suxinjs@163.com Qi Zhang, Department of Respiratory and Critical Care Medicine, Xuzhou Central Hospital, Xuzhou, Jiangsu, 221000, People’s Republic of China, Email zhangqi1107@126.comBackground: tNGS and mNGS are valuable tools for diagnosing pathogens in lower respiratory tract infections (LRTIs), which subsequently influence treatment strategies. However, the impact of tNGS and mNGS on antimicrobial stewardship in patients with LRTIs remains unclear.Methods: Patients diagnosed with LRTIs who underwent tNGS or mNGS between June 2021 and January 2024 were included. Patients who underwent both tNGS and conventional microbiologic tests (CMTs) were grouped into the tNGS group, the others were divided into the mNGS group. Then, the diagnostic efficacy of tNGS and mNGS was compared, along with their impact on antimicrobial management and clinical outcomes.Results: 548 patients with an initial diagnosis of LRTIs who underwent tNGS or mNGS were evaluated. Finally, 321 patients were analyzed, with 117 patients in tNGS group and 204 patients in mNGS group. The overall pathogen detection rates for tNGS and mNGS were 89.74% and 89.71% (P=0.991). The distribution of detected pathogens was similar between tNGS and mNGS, with bacteria being the predominant microorganisms. The proportions of patients who underwent antimicrobial agent changes and received targeted therapy were not significantly different between tNGS and mNGS groups (P=0.270; P=0.893). Additionally, no significant differences were noted in the rates of antibiotic de-escalation, escalation, or changes in the opposite direction (all P> 0.05). The same results was observed in the proportions of patients with addition or reductions in antiviral, antifungal, and antibacterial agents (all P> 0.05). Hospital stays, improvement rate and mortality rate were also similar (all P> 0.05).Conclusion: tNGS and mNGS demonstrate comparable overall pathogen yield rates in patients with LRTIs. Furthermore, tNGS is also comparable to mNGS in terms of adjusting antimicrobial treatments and clinical outcomes, tNGS meets the clinical needs of most patients with LRTIs and can be firstly used for these patients.Keywords: tNGS, mNGS, antimicrobial management, lower respiratory tract infectionhttps://www.dovepress.com/comparison-of-the-impact-of-tngs-with-mngs-on-antimicrobial-management-peer-reviewed-fulltext-article-IDRtngsmngsantimicrobial managementlower respiratory tract infection. |
| spellingShingle | Li D Li Q Huang Z Wu W Fan X Liu J Li R Zhang Q Su X Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study Infection and Drug Resistance tngs mngs antimicrobial management lower respiratory tract infection. |
| title | Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study |
| title_full | Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study |
| title_fullStr | Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study |
| title_full_unstemmed | Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study |
| title_short | Comparison of the Impact of tNGS with mNGS on Antimicrobial Management in Patients with LRTIs: A Multicenter Retrospective Cohort Study |
| title_sort | comparison of the impact of tngs with mngs on antimicrobial management in patients with lrtis a multicenter retrospective cohort study |
| topic | tngs mngs antimicrobial management lower respiratory tract infection. |
| url | https://www.dovepress.com/comparison-of-the-impact-of-tngs-with-mngs-on-antimicrobial-management-peer-reviewed-fulltext-article-IDR |
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