Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging
The positive hemodynamic response (PHR) during stimulation often co-occurs with a strong, sustained negative hemodynamic response (NHR). However, the characteristics and neurophysiological mechanisms of the NHR, especially in regions distal to the PHR, remain incompletely understood. Using intrinsic...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923024002831 |
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| author | Zhen Li Lihua He Limin Peng Xuan Zhu Ming Li Dewen Hu |
| author_facet | Zhen Li Lihua He Limin Peng Xuan Zhu Ming Li Dewen Hu |
| author_sort | Zhen Li |
| collection | DOAJ |
| description | The positive hemodynamic response (PHR) during stimulation often co-occurs with a strong, sustained negative hemodynamic response (NHR). However, the characteristics and neurophysiological mechanisms of the NHR, especially in regions distal to the PHR, remain incompletely understood. Using intrinsic optical imaging (OI) and two-photon imaging, we observed that forelimb electrical stimulation evoked strong PHR signals in the forelimb region of the primary somatosensory cortex (S1FL). Meanwhile, NHR signals primarily appeared in the primary visual cortex (V1), with a delayed onset and lower amplitude relative to the PHR signals. Additionally, stimulation led to a reduction in cerebral blood flow (CBF) in the NHR region. Notably, there was an overall suppression of the calcium response in the NHR region, although a small proportion (14 %) of neurons exhibited concurrent activation. Axon tracing revealed cortico-cortical projections from S1FL to V1. These findings suggest that neuronal deactivation significantly contributes to the origin of the NHR, offering additional insights into the specific inhibitory mechanisms underlying the NHR. |
| format | Article |
| id | doaj-art-ac8a9a40c14b4a68a1ce3d12ee3799da |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj-art-ac8a9a40c14b4a68a1ce3d12ee3799da2025-01-10T04:36:57ZengElsevierBrain Research Bulletin1873-27472025-01-01220111149Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imagingZhen Li0Lihua He1Limin Peng2Xuan Zhu3Ming Li4Dewen Hu5College of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaCollege of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaCollege of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaCollege of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaCorresponding authors.; College of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaCorresponding authors.; College of Intelligence Science and Technology, National University of Defense Technology, Changsha, ChinaThe positive hemodynamic response (PHR) during stimulation often co-occurs with a strong, sustained negative hemodynamic response (NHR). However, the characteristics and neurophysiological mechanisms of the NHR, especially in regions distal to the PHR, remain incompletely understood. Using intrinsic optical imaging (OI) and two-photon imaging, we observed that forelimb electrical stimulation evoked strong PHR signals in the forelimb region of the primary somatosensory cortex (S1FL). Meanwhile, NHR signals primarily appeared in the primary visual cortex (V1), with a delayed onset and lower amplitude relative to the PHR signals. Additionally, stimulation led to a reduction in cerebral blood flow (CBF) in the NHR region. Notably, there was an overall suppression of the calcium response in the NHR region, although a small proportion (14 %) of neurons exhibited concurrent activation. Axon tracing revealed cortico-cortical projections from S1FL to V1. These findings suggest that neuronal deactivation significantly contributes to the origin of the NHR, offering additional insights into the specific inhibitory mechanisms underlying the NHR.http://www.sciencedirect.com/science/article/pii/S0361923024002831Negative hemodynamic responses (NHR)Intrinsic optical imaging (OI)Two-photon imagingNeuronal deactivationInhibitory mechanisms |
| spellingShingle | Zhen Li Lihua He Limin Peng Xuan Zhu Ming Li Dewen Hu Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging Brain Research Bulletin Negative hemodynamic responses (NHR) Intrinsic optical imaging (OI) Two-photon imaging Neuronal deactivation Inhibitory mechanisms |
| title | Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging |
| title_full | Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging |
| title_fullStr | Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging |
| title_full_unstemmed | Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging |
| title_short | Negative hemodynamic response in the visual cortex: Evidence supporting neuronal origin via hemodynamic observation and two-photon imaging |
| title_sort | negative hemodynamic response in the visual cortex evidence supporting neuronal origin via hemodynamic observation and two photon imaging |
| topic | Negative hemodynamic responses (NHR) Intrinsic optical imaging (OI) Two-photon imaging Neuronal deactivation Inhibitory mechanisms |
| url | http://www.sciencedirect.com/science/article/pii/S0361923024002831 |
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