Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey
<b>Aim:</b> The present study was on the detection of Salmonella spp. and two important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in chicken meat and giblets and also determination of antimicrobial resistance of the isolates.<p> <b>Materials and Methods:</b...
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Selcuk University Press
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| author | Arife Ezgi Telli Yusuf Biçer Hatice Ahu Kahraman Nihat Telli Yusuf Doğruer |
| author_facet | Arife Ezgi Telli Yusuf Biçer Hatice Ahu Kahraman Nihat Telli Yusuf Doğruer |
| author_sort | Arife Ezgi Telli |
| collection | DOAJ |
| description | <b>Aim:</b> The present study was on the detection of Salmonella spp. and two
important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in
chicken meat and giblets and also determination of antimicrobial resistance
of the isolates.<p>
<b>Materials and Methods:</b> In this study, livers (n=40), gizzards (n=40),
hearts (n=30), skins (n=30), drumsticks (n=10) and wings (n=20) were
collected from supermarkets and butcher shops in Konya, Turkey. The
samples were analyzed by Classical Cultural Technique. Molecular
confirmation of the suspicious colonies was carried out using Inv-A gene-
based PCR. Flic-C and IE-1 primers were used by duplex PCR for S.
Thyphimurium and S. Enteritidis respectively. Antibiotic resistance of
the isolates was determined by the disk diffusion method.<p>
<b>Results:</b> Forty-three (25.29 %) of 170 samples were positive for Salmonella
spp. According to the d-PCR assay, neither S. Thyphimurium nor
S. Enteritidis was not detected. The resistance to clindamycin, oxacillin,
teicoplanin were evident 100 % and resistance to vancomycin (79.1
%), erythromycin (79.1 %), nalidixic acid (65.1 %), penicillin G (60.5
%) cephalothin (48.8 %), sulfamethoxazole-trimethoprim (37.2 %),
tetracycline (37.2 %), ampicillin (23.3 %), kanamycin (18.6 %), chloramphenicol
(11.6 %) amikacin, cephazoline, ciprofloxacin, gentamycin
(4.7 %) was also detected. All isolates were susceptible to amoxicillin/
clavulanic acid and cefixime.<p>
<b>Conclusion:</b> The results indicated that S. Enteritidis and S. Typhimurium
were not identified and it was considered satisfactory in terms of
public health. It should be still important to note the studies to identify
species with lower pathogenic incidences for legal legislation. Furthermore,
even the most common pathogenic species cannot be detected,
the results of antibiotic resistance in isolates were noteworthy for antibiotic
surveillance database. |
| format | Article |
| id | doaj-art-abba31abf07b44c3ae57c50f9c90fd00 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-abba31abf07b44c3ae57c50f9c90fd002025-01-03T01:36:16ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19533431641701215Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkeyArife Ezgi Telli0Yusuf Biçer1Hatice Ahu Kahraman2Nihat Telli3Yusuf Doğruer4Selcuk University Faculty of Veterinary Medicine Department of Food Hygiene and Technology KonyaSelcuk University Faculty of Veterinary Medicine Department of Food Hygiene and Technology KonyaMehmet Akif Ersoy University Faculty of Veterinary Medicine Department of Food Hygiene and Technology BurdurKonya Technical University, Food Technology, Konya, TürkiyeSelcuk University Faculty of Veterinary Medicine Department of Food Hygiene and Technology Konya<b>Aim:</b> The present study was on the detection of Salmonella spp. and two important Salmonella serotypes (S. Thyphimurium and S. Enteritidis) in chicken meat and giblets and also determination of antimicrobial resistance of the isolates.<p> <b>Materials and Methods:</b> In this study, livers (n=40), gizzards (n=40), hearts (n=30), skins (n=30), drumsticks (n=10) and wings (n=20) were collected from supermarkets and butcher shops in Konya, Turkey. The samples were analyzed by Classical Cultural Technique. Molecular confirmation of the suspicious colonies was carried out using Inv-A gene- based PCR. Flic-C and IE-1 primers were used by duplex PCR for S. Thyphimurium and S. Enteritidis respectively. Antibiotic resistance of the isolates was determined by the disk diffusion method.<p> <b>Results:</b> Forty-three (25.29 %) of 170 samples were positive for Salmonella spp. According to the d-PCR assay, neither S. Thyphimurium nor S. Enteritidis was not detected. The resistance to clindamycin, oxacillin, teicoplanin were evident 100 % and resistance to vancomycin (79.1 %), erythromycin (79.1 %), nalidixic acid (65.1 %), penicillin G (60.5 %) cephalothin (48.8 %), sulfamethoxazole-trimethoprim (37.2 %), tetracycline (37.2 %), ampicillin (23.3 %), kanamycin (18.6 %), chloramphenicol (11.6 %) amikacin, cephazoline, ciprofloxacin, gentamycin (4.7 %) was also detected. All isolates were susceptible to amoxicillin/ clavulanic acid and cefixime.<p> <b>Conclusion:</b> The results indicated that S. Enteritidis and S. Typhimurium were not identified and it was considered satisfactory in terms of public health. It should be still important to note the studies to identify species with lower pathogenic incidences for legal legislation. Furthermore, even the most common pathogenic species cannot be detected, the results of antibiotic resistance in isolates were noteworthy for antibiotic surveillance database.http://eurasianjvetsci.org/pdf.php3?id=1215antibiotic resistance; chicken; s. enteritidis; s. typhimurium; salmonella spp |
| spellingShingle | Arife Ezgi Telli Yusuf Biçer Hatice Ahu Kahraman Nihat Telli Yusuf Doğruer Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey Eurasian Journal of Veterinary Sciences antibiotic resistance; chicken; s. enteritidis; s. typhimurium; salmonella spp |
| title | Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey |
| title_full | Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey |
| title_fullStr | Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey |
| title_full_unstemmed | Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey |
| title_short | Presence and antibiotic resistance of salmonella spp. isolated from chicken meat and giblets consumed in konya, turkey |
| title_sort | presence and antibiotic resistance of salmonella spp isolated from chicken meat and giblets consumed in konya turkey |
| topic | antibiotic resistance; chicken; s. enteritidis; s. typhimurium; salmonella spp |
| url | http://eurasianjvetsci.org/pdf.php3?id=1215 |
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