Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia
Biofilm, intricate microbial communities adhering to submerged surface, pose a significant challenge across various sectors. Conventional approaches relying on harsh chemical or mechanical removal have drawback. Marine organisms like sponges, with their diverse secondary metabolites, offer a promisi...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2024-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01008.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526818854666240 |
|---|---|
| author | Gazali Mohamad Nur Izzah Alissa Wan Rosli Wan Sarah Mohd Zahidi Siti Amira Jamaludin Nur Bakar Kamariah Saidin Jasnizat |
| author_facet | Gazali Mohamad Nur Izzah Alissa Wan Rosli Wan Sarah Mohd Zahidi Siti Amira Jamaludin Nur Bakar Kamariah Saidin Jasnizat |
| author_sort | Gazali Mohamad |
| collection | DOAJ |
| description | Biofilm, intricate microbial communities adhering to submerged surface, pose a significant challenge across various sectors. Conventional approaches relying on harsh chemical or mechanical removal have drawback. Marine organisms like sponges, with their diverse secondary metabolites, offer a promising avenue for developing sustainable solutions. This study investigated the antibiofilm potential of crude extracts from four marine sponges (Stylissa carteri, Haliclona amboinensis, Hymeniacidon sp., and Xestospongia testudinaria) against Vibrio parahaemolyticus and Pseudomonas aeruginosa biofilms. Crystal Violet staining quantified biofilm formation and removal. Well diffusion assays assessed antibacterial activity. Haliclona amboinensis and Hymeniacidon sp. extracts displayed antibiofilm effects against V. parahaemolyticus biofilm. The IC50 values for the activity were 0.1 mg/mL and 0.19 mg/mL, respectively. Notably, the Hymeniacidon sp. extract exhibited bactericidal activity whereas H. amboinensis lacked this effect. Interestingly, both extracts effectively removed pre-formed V. parahaemolyticus biofilms against P. aeruginosa, H. amboinensis displayed antibiofilm activity (IC50 = 2.8 mg/mL) without bactericidal effects. However, this extract did not impact pre-established P. aeruginosa biofilms. The study suggests H. amboinensis is a promising source for new antibiofilm agents. It effectively inhibits biofilm formation without killing bacteria. Further research is needed to identify the active compounds and their mechanisms for developing targeted therapies. |
| format | Article |
| id | doaj-art-aa93ad25272145f382e554c69a178932 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-aa93ad25272145f382e554c69a1789322025-01-16T11:19:24ZengEDP SciencesBIO Web of Conferences2117-44582024-01-011470100810.1051/bioconf/202414701008bioconf_eafta2024_01008Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, MalaysiaGazali Mohamad0Nur Izzah Alissa Wan Rosli Wan1Sarah Mohd Zahidi Siti2Amira Jamaludin Nur3Bakar Kamariah4Saidin Jasnizat5Institute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia TerengganuFaculty of Science and Marine Environment, Universiti Malaysia TerengganuFaculty of Science and Marine Environment, Universiti Malaysia TerengganuInstitute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia TerengganuInstitute of Climate Adaptation and Marine Biotechnology, Universiti Malaysia TerengganuFaculty of Science and Marine Environment, Universiti Malaysia TerengganuBiofilm, intricate microbial communities adhering to submerged surface, pose a significant challenge across various sectors. Conventional approaches relying on harsh chemical or mechanical removal have drawback. Marine organisms like sponges, with their diverse secondary metabolites, offer a promising avenue for developing sustainable solutions. This study investigated the antibiofilm potential of crude extracts from four marine sponges (Stylissa carteri, Haliclona amboinensis, Hymeniacidon sp., and Xestospongia testudinaria) against Vibrio parahaemolyticus and Pseudomonas aeruginosa biofilms. Crystal Violet staining quantified biofilm formation and removal. Well diffusion assays assessed antibacterial activity. Haliclona amboinensis and Hymeniacidon sp. extracts displayed antibiofilm effects against V. parahaemolyticus biofilm. The IC50 values for the activity were 0.1 mg/mL and 0.19 mg/mL, respectively. Notably, the Hymeniacidon sp. extract exhibited bactericidal activity whereas H. amboinensis lacked this effect. Interestingly, both extracts effectively removed pre-formed V. parahaemolyticus biofilms against P. aeruginosa, H. amboinensis displayed antibiofilm activity (IC50 = 2.8 mg/mL) without bactericidal effects. However, this extract did not impact pre-established P. aeruginosa biofilms. The study suggests H. amboinensis is a promising source for new antibiofilm agents. It effectively inhibits biofilm formation without killing bacteria. Further research is needed to identify the active compounds and their mechanisms for developing targeted therapies.https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01008.pdf |
| spellingShingle | Gazali Mohamad Nur Izzah Alissa Wan Rosli Wan Sarah Mohd Zahidi Siti Amira Jamaludin Nur Bakar Kamariah Saidin Jasnizat Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia BIO Web of Conferences |
| title | Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia |
| title_full | Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia |
| title_fullStr | Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia |
| title_full_unstemmed | Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia |
| title_short | Investigation of Antibiofilm Activities from Marine Sponges’ Crude Extract of Pulau Bidong, Terengganu, Malaysia |
| title_sort | investigation of antibiofilm activities from marine sponges crude extract of pulau bidong terengganu malaysia |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01008.pdf |
| work_keys_str_mv | AT gazalimohamad investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia AT nurizzahalissawanrosliwan investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia AT sarahmohdzahidisiti investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia AT amirajamaludinnur investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia AT bakarkamariah investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia AT saidinjasnizat investigationofantibiofilmactivitiesfrommarinespongescrudeextractofpulaubidongterengganumalaysia |