Effects of dietary lipid source and level on growth performance, antioxidant capacity, and hepatic metabolism in marine teleost Trachinotus ovatus: Insights from fatty acid composition
This study investigated the effects of two dietary lipid sources (BO1: optimized fatty acid profile for Trachinotus ovatus, BO2: commonly used in feed for T. ovatus) at varying dietary lipid levels (13 %, 16 %, 19 %) on growth performance, antioxidant capacity, and hepatic metabolism in T. ovatus. S...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425002972 |
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| Summary: | This study investigated the effects of two dietary lipid sources (BO1: optimized fatty acid profile for Trachinotus ovatus, BO2: commonly used in feed for T. ovatus) at varying dietary lipid levels (13 %, 16 %, 19 %) on growth performance, antioxidant capacity, and hepatic metabolism in T. ovatus. Six isonitrogenous (45 %) diets were formulated: D13/D16/D19 (BO1-based) and T13/T16/T19 (BO2-based). A total of 540 juveniles (7.63 ± 0.20 g) were evenly distributed across 18 cages (1 m × 1 m × 2 m), with three replicate cages per diet, and fed twice daily to satiety for 9 weeks under natural conditions (25.0–27.8 °C, 32–33 ‰ salinity). BO1-fed fish demonstrated superior growth metrics (weight gain rate, feed intake), enhanced hepatic antioxidant capacity (elevated SOD activity, T-AOC; reduced MDA), and lower hepatic lipid and linoleic acid accumulation compared to BO2 groups (P < 0.05). At 19 % lipid inclusion, BO1 maintained growth performance while improving antioxidant status, whereas BO2 reduced survival rate (98.89 % vs. 96.67 %) and feed intake (87.24 vs. 75.35 g/fish) and increased hepatic lipid peroxidation. Metabolomic profiling revealed that BO1, at 19 % dietary lipid, upregulated hepatic metabolites associated with gamma-aminobutyric acid (GABA) metabolism (e.g., GABA, succinic semialdehyde) and tricarboxylic acid (TCA) cycle intermediates (e.g., succinate, cis-aconitate), corroborated by elevated expression of associated genes (e.g., gababr, gat2, cs) (P < 0.05). In vitro hepatocyte assays confirmed fatty acid composition-dependent regulation of these pathways. These findings establish BO1 as a superior lipid source supporting 19 % dietary lipid inclusion through metabolic modulation of GABA and energy metabolism pathways. |
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| ISSN: | 2352-5134 |