Development and validation of a droplet digital PCR method for quantifying lentiviral vector infectious titer

Development and validation of a droplet digital PCR method for quantifying lentiviral vector infectious titer

Lentiviruses, with their high transduction efficiency and gene expression levels, are widely used as gene delivery vectors in the development of chimeric antigen receptor T cells (CAR-T) and other genetically modified cell therapies. Accurate determination of the lentiviral vector infectious titer i...

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Bibliographic Details
Main Authors: Xueling Wu, Xiaoya Zhou, Yueming Wang, Jian Wu, Qian Liang, Xu Yang, Kehua Zhang, Shufang Meng
Format: Article
Language:English
Published: Elsevier 2024-10-01
Series:Heliyon
Subjects:
Lentiviral vectors
Infectious titer
Digital droplet polymerase chain reaction (ddPCR)
Digestive enzymes
Method validation
Online Access:http://www.sciencedirect.com/science/article/pii/S2405844024145434
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